Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0220723 (PCA)
 4,687 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We investigated the onset and duration of DNA synthesis and differentiation of muscle precursor cells in the early stage in muscle regeneration after bupivacaine induced muscle necrosis using monoclonal anti-PCA and anti-desmin antibodies. The first positive PCNA reaction indicating the commencement of proliferating cycle of muscle precursor cells appeared at 24 hours after injury, and were most prominent at 60-72 hours after injury. Thereafter the reaction for PCNA disappeared as the maturation of regenerated myofibers progressed. Some nuclei of regenerated myotubes in the early stage were positive for PCNA contrary to the generally accepted concept that myonuclei after fusion were out of cell cycle. The first desmin expression was observed in mononuclear presumptive myoblasts at 48 hours after injury, some of which were also positive for PCNA in the double immunostained sections for desmin and PCNA. This result suggested that differentiation of presumptive myoblasts could already initiate during their proliferative phase.
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PMID:Immunohistochemical analysis of myoblast proliferation and differentiation in experimental skeletal muscle regeneration. 810 86

Antibody producing B-cells are an essential component of the immune system. In contrast to human and mice where B-cells develop in the bone marrow, chicken B-cells develop in defined stages in the bursa of Fabricius, a gut associated lymphoid tissue. In order to gain a better understanding of critical biological processes like immigration of B-cell precursors into the bursa anlage, their differentiation and final emigration from the bursa we analyzed the proteome dynamics of this organ during embryonic and posthatch development. Samples were taken from four representative developmental stages (embryonic day (ED) 10, ED18, day 2, and day 28) and compared in an extensive 2D DIGE approach comprising six biological replicates per time point. Cluster analysis and PCA demonstrated high reliability and reproducibility of the obtained data set and revealed distinctive proteome profiles for the selected time points, which precisely reflect the differentiation processes. One hundred fifty three protein spots with significantly different intensities were identified by MS. We detected alterations in the abundance of several proteins assigned to retinoic acid metabolism (e.g. retinal-binding protein 5) and the actin-cytoskeleton (e.g. vinculin and gelsolin). By immunohistochemistry, desmin was identified as stromal cell protein associated with the maturation of B-cell follicles. Strongest protein expression difference (10.8-fold) was observed for chloride intracellular channel 2. This protein was thus far not associated with B-cell biology but our data suggest an important function in bursa B-cell development.
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PMID:2D DIGE analysis of the bursa of Fabricius reveals characteristic proteome profiles for different stages of chicken B-cell development. 2313 93