Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0220723 (PCA)
 4,687 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The antigencity of CS-1170, a newly developed semi-synthetic cephamycin, and it's cross-reactivity with beta-lactam antibiotics were studied. The antigencity was confirmed by the following tests: 1)the passive hemagglutination test with anti-CS-1170 antisera of guinea pigs immunized with CS-1170 plus Freund complete adjuvant, 2)lymphocyte proliferation response in vitro stimulated with this drug in the guinea pigs, 3) the PCA test using mice immunized with CS-1170-protein conjugates. The antibody to CS-1170 appears to be directed to the acyl side chain because cyanomethylthioacetylglycine, an univalent acyl side chain of the CS-1170, can significantly cross-react with the antibody. A methoxy group on the C-7 alpha-position of the antibiotic plays no important part for the antigenic specificity of the molecule. The cross-reactivity of CS-1170 with cefazolin, cephalothin, benzylpenicillin and ampicillin was only to a minimal extent in the passive hemagglutination test. The cross-reactivity of CS-1170 and the related antibiotics was not observed among them in the PCA system with IgE anti-CS-1170. These findings support a conclusion that CS-1170 is one of beta-lactam antibiotic with an immunologically minimal cross-reactivity to the related antibiotics.
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PMID:Immunological studies on CS-1170, a new semisynthetic cephamycin antibiotic. 8 34

The effects of the different acyl side chains of azidocillin, ampicillin and benzylpenicillin on the immunogenic potency of penicilloylated antigens as well as on the specificity of the developed antibodies were investigated in CBA mice. The antigens used were penicilloylated bovine gammaglobulin (BGG), bovine serum albumin (BSA) and human serum albumin (HSA). Immunization was performed with injection of high doses of antigens together with adjuvant or by injection of minute amounts of antigen over periods of 10 days. IgE antibodies were recorded with PCA in rats and IgG antibodies were measured with a double antibody assay. The nature of the carrier as well as the number of epitopes was found to influence the development of antibodies irrespective of the immunization schedule used. The immunogenic activity of the PO-BSA antigens was related to the epitope density. The PO-BSA antigens were, in contrast to the PO-BGG antigens, weak immunogens in the CBA mice. The acyl side chains of the different penicillins influenced the induction and specificity of the IgE antibody responses obtained after daily treatment.
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PMID:Antigens in penicillin allergy. V. On the relative allergenic potency of antigens carrying penicilloyl determinants derived from azidocillin, ampicillin and benzylpenicillin. 615 79

BALB/C mice were immunized with conjugate of benzylpenicillin or ampicillin with Ascaris suum extract. The mice developed IgE antibodies to penicillin, which were found to react with commercially available penicillin preparations in the PCA system. Elimination of polymerized penicillin by Sephadex chromatography from tested preparations could not diminish their activities to elicit PCA. High pressure liquid chromatographic (HPLC) separation of PcG preparation yielded fractions unable to elicit PCA. On the other hand, all fractions from ABPC retained the activity even after the HPLC purification. A comparative estimation of cross-reactivity of IgE and IgE antipenicillin antibodies showed that IgE antibodies cross-reacted with a variety of beta-lactam antibiotics in a high degree.
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PMID:Antigenicity of beta-lactam antibiotic preparations: production of IgE antibodies to beta-lactam antibiotic and their cross-reaction within the antibiotic group. 617 50

In the present study, the correlation between mouse PLNA results and those obtained from GP-PCA and from GP-ASA reactions for sodium 2,4,6-trinitrobenzenesulfonate dihydrate (TNBS), penicillin G and cephalothin was investigated. Next, various drugs were tested using the mouse PLNA to study whether PLN reactivity could be related to the potential of the compounds to induce allergic and autoimmune disorders in humans. The parameter of the PLNA was determined by the PLN cellularity index in BALB/c and A/J mice treated with a single subcutaneous injection of compounds. Hartley guinea pigs were immunized subcutaneously with the compounds without adjuvant, and then the GP-PCA and GP-ASA reactions were assessed. The examinations using mice and guinea pigs showed that mouse PLN responses to TNBS, penicillin G and cephalothin correlated with the allergenicity responses obtained in the GP-PCA reaction to three compounds. In the mouse PLNA, ten drugs considered to be well-known inducers of allergic side-effects in humans (i.e., penicillin G, cephalothin, ampicillin, carbenicillin, cefazolin, cefotaxime, streptomycin, 4-aminoantipyrine, chlorhexidine and sulfamethoxazole) caused increases in PLN cellularity indices as well. These results indicate that the PLNA may be useful as a short-term and simple test system for detecting low-molecular drugs exhibiting allergenicity potential.
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PMID:Evaluation of allergenic potential of low-molecular compounds by mouse popliteal lymph node assay. 992 46

Five brands of French bottled mineral water were analyzed by heterotrophic plate counts (HPC) and for the presence of multiple antibiotic resistant bacteria. HPC at 22 degrees C were around 10(4) colony forming units ml(-1) on R2A medium. Enumeration on PCA/10, MH, and especially PCA and King B media was less efficient. At 37 degrees C, HPC were two to three orders of magnitude less than at 22 degrees C. Moreover, phenotypic diversity (7 to 15 phenotypes) was optimal on R2A incubated at 22 degrees C. All isolates were identified as non-fermentative Gram-negative rods and 75% were non-identifiable with the API 20NE system. Stenotrophomonas maltophilia and fluorescent Pseudomonas were isolated on VIA and CFC selective agar media, respectively. Burkholderia cepacia strains were not isolated on BCSA medium. The species S. maltophilia was found in 33%, 28%, and 11% of sample from springs A, D, and E, respectively. Independent of brand, isolates from HPC media were less efficient to achieve confluent growth in 18 h on MH at 30 or 37 degrees C (0 to 40%) than isolates from selective media (28 to 63%). Seventy percent of the total isolates from dominant microflora (1-5 x 10(3) CFU ml(-1) on HPC media) were resistant against two or four antibiotics. The antibiotics concerned were principally aztreonam, ampicillin, and nalidixic acid. The remaining dominant bacteria showed a 6-9 multiple antibiotic resistant (MAR) pattern. All isolates were susceptible to newer antimicrobial agents. Owing to their low nutrient and temperature requirements, these isolates are unlikely to cause concern to public heath. Fifty percent of strains isolated from selective media (non-dominant microflora, 4-40 CFU l(-1)) showed a 10-18 MAR pattern and 33%, identified as S. maltophilia, a 20-27 MAR pattern. However, minocycline was effective against all isolates. Owing to its low concentration, colonization of human intestine by MAR S. maltophilia is unlikely.
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PMID:Occurrence and Multiple Antibiotic Resistance Profiles of Non-fermentative Gram-Negative Microflora in Five Brands of Non-carbonated French Bottled Spring Water. 1088 37

This paper presents a methodology based on multivariate data analysis for characterizing potential source contributions of emerging contaminants (ECs) detected in 26 river water samples across multi-scape regions during dry and wet seasons. Based on this methodology, we unveil an approach toward potential source contributions of ECs, a concept we refer to as the "Pharmaco-signature." Exploratory analysis of data points has been carried out by unsupervised pattern recognition (hierarchical cluster analysis, HCA) and receptor model (principal component analysis-multiple linear regression, PCA-MLR) in an attempt to demonstrate significant source contributions of ECs in different land-use zone. Robust cluster solutions grouped the database according to different EC profiles. PCA-MLR identified that 58.9% of the mean summed ECs were contributed by domestic impact, 9.7% by antibiotics application, and 31.4% by drug abuse. Diclofenac, ibuprofen, codeine, ampicillin, tetracycline, and erythromycin-H2O have significant pollution risk quotients (RQ>1), indicating potentially high risk to aquatic organisms in Taiwan.
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PMID:Source apportionment and risk assessment of emerging contaminants: an approach of pharmaco-signature in water systems. 2601 50