Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0220723 (PCA)
 4,687 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Two allergen pools of commercial detergent enzymes were prepared as skin test reagents: (1) Carlsberg type, composed of three products containing subtilopeptidase A, and (2) BPN type, composed of two products containing subtilopeptidase B and alpha-amylase. In 100 non-exposed controls a reaction suggesting primary irritancy was found at protein concentrations greater than 1 microng/ml intradermally or 1 mg/ml by prick test. Intradermally at 10 microng/ml weals were accompained by less pronounced flare reactions than observed in specifically sensitized enzyme workers. At 100 micronh/ml the reactions were like strong specific reactions. Galse positive prick test reactions occurred irregularly at 10 mg/ml. In 100 sensitized enzyme workers, reactions were elicited at concentrations from 1-0 to 10(-5) microng/ml intradermally and from 1000 to 1 micron by prick test. Intradermal and prick tests correlated well (r=0-84, P less than 0-001). Ratings of symptom severity upon exposure obtained from questionnaires were significantly correlated with skin test reactivity (P less than 0-01). RAST performed on sera collected simultaneously also correlated significantly with symptom scores. PCA tests in monkeys were less sensitive. Standardized test reagents allow diagnostic skin testing by either intradermal or prick test in B. subtilis enzyme sensitive patients. A clear distinction between primary irritant reactions and true sensitization was made on the basis of the concentration required to elicit a reaction.
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PMID:Diagnostic tests in the skin and serum of works sensitized to Bacillus subtilis enzymes. 40

Two stable lines of IgA lambda-producing plasma cells (KHM-1A and KHM-1B) that were free of the Epstein-Barr virus were established from a patient with multiple myeloma complicated by hyperamylasemia. Surface marker studies of the two cell lines showed that the cells had no surface immunoglobulins but were positive for cytoplasmic immunoglobulins (IgA lambda) and for HLA-DR and PCA-1. Secretion of IgA monoclonal immunoglobulin by the two lines was detected by a plaque-forming cell assay and by an enzyme-linked immunosorbent assay of culture media. KHM-1B cells also secreted alpha-amylase, but no such activity was detected in the culture-conditioned supernatant fluid of KHM-1A.
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PMID:Establishment and characterization of an amylase-producing human myeloma cell line. 245 53