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Query: UMLS:C0205700 (ash)
 15,125 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Earlier studies have shown that a substance(s) released from the egg jelly of the toad Bufo arenarum is required for fertilization. In this paper some properties of this diffusible factor were further examined, and a procedure was designed for its isolation from crude egg extracts. The active component is soluble in water and ethanol, and insoluble in chloroform, ether and n-butanol. The biological activity is stable to liophylization and to heat, and remains unaffected after trypsin treatment. In contrast, it is impaired after treatment with ethyl acetate, 0.1 N HCl or chloroform, and is completely destroyed after converting the diffusible factor into ash. Data are presented showing that the recovery of fertilizability of extracted eggs in the bioassay system as carried out under present conditions, cannot be ascribed to a pH alteration of the insemination medium. This lends further support to the view that diffusible factor activity is not mediated through a pH effect. The factor was purified by gel chromatography coupled with desalting and paper chromatography. The active molecule is of low molecular weight and appears associated with a high pH ninhydrin-positive fraction.
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PMID:Properties and isolation of the diffusible factor involved in Bufo arenarum fertilization. 11 83

A rapid direct dilution procedure for the estimation of soluble zirconium and a fusion procedure for the determination of total zirconium (soluble and insoluble forms) in cream base concentrates prepared from antiperspirant aerosols are described. The direct dilution procedure involves extraction of soluble zirconium with HCl (55 + 45). The filtered extract is reacted with alizarin red S to form a stable colored complex which is measured spectrophotometrically. The fusion procedure involves ashing the aerosol concentrate followed by fusion of the ash with potassium pyrosulfate to form an acid-soluble melt. Zirconium is precipitated from solution as the hydroxide and washed to eliminate interfering ions, particularly sulfate. After redissolving in HCl (55 + 45) and reacting with alizarin red S, total zirconium is measured. Zirconyl chloride octahydrate, assayed gravimetrically by hydroxide precipitation and conversion to the oxide, is used as the zirconium reference standard. Concentration range of zirconium measured was 200-500 mug/100 ml. Recoveries of standard zirconium added to commercial aerosols labeled to contain aluminum and zirconyl hydroxychlorides ranged from 97 to 101% by the fusion procedure. Analysis of these aerosols by direct dilution gave generally slightly lower results than by fusion. It is recommended that the procedures be collaboratively studied after further testing of their general applicability to a variety of drugs and cosmetics.
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PMID:Colorimetric determination of zirconium in antiperspirant aerosols. 93 50

A method is described for the preparation of bovine ruminal contents, duodenal digesta, and feces for analysis of Co, Cr, La, Sm, Eu, and Yb by direct current plasma emission spectroscopy. Ground, dried sample was refluxed in concentrated nitric acid until 2 to 3 ml of acid remained. The ash was redissolved in 6N HCl. Lithium hydroxide was added to suppress ionization, and the samples were diluted with distilled water for analysis. Element recovery, expressed as the ratio of element recovered from digesta samples augmented prior to ashing compared with those augmented after sample digestion, ranged from 71.5 to 100.5% and was affected by digesta type. Addition of 1000 ppm of Li to the sample matrix improved recovery of Co and La from 93.4 and 83.3% to 97.8 and 98.3%, respectively. Lithium did not affect recovery of the other elements. Type of digesta matrix affected emission intensity. Addition of 1000 ppm of Li to the sample matrices enhanced signal output in the digesta matrices by 25 to 30% for each element. Lithium tended to minimize differences in signal output caused by the organic matrix but did not eliminate them. Increasing Li concentration from 1000 to 4000 ppm did not increase signal output or completely buffer out the higher emission intensity associated with the organic matrix. Sensitivity of analysis of Co, Cr, and rare earth elements was comparable with or better than sensitivities reported with neutron activation analysis.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Determination of markers in digesta and feces by direct current plasma emission spectroscopy. 140 69

Relative biological values (BV) of 36 feed phosphates were determined with female turkeys in bioassays of 21-day duration using three response criteria: weight gain, tibia ash percentage, and gain:feed ratio. Calcium phosphate, dibasic dihydrate (United States Pharmacopeia) was the reference standard. Nine mono-dicalcium phosphates (M-DCP, 21.0% phosphorus), 13 di-monocalcium phosphates (D-MCP, 18.5% phosphorus), and 14 defluorinated phosphates (DFP, 18.0% phosphorus) were evaluated. The average relative BV for M-DCP, D-MCP, and DFP samples were 97.6, 94.6, and 90.8%, respectively. Solubility of phosphates was determined by four recognized methods. The solvents were water, .4% HCl, 2.0% citric acid (CA), and neutral ammonium citrate (NAC). Water solubility of M-DCP samples was greater (67.5%) than that of D-MCP (38.8%) and DFP (8.9%) samples. Correlation of water solubility of phosphates to their relative BV was quite low, and water solubility was a poor indicator of BV. When .4% HCl was the solvent, correlation coefficients (r) were .55, .33, and .72 for M-DCP, D-MCP, and DFP, respectively. Based on these results and prediction equations, .4% HCl solubility would be inappropriate for estimating BV of M-DCP and D-MCP samples. Solubility of feed phosphates (mainly D-MCP and DFP) in 2.0% CA or NAC was positively correlated with BV; the r values were .87 to .95. Both of these solubility tests provided a good index of BV. However, it would seem inappropriate and risky to replace bioassays totally with these tests. Feed phosphate users could perform either the 2.0% CA or NAC solubility test easily as a screen for BV along with other quality control procedures (i.e., phosphorus, calcium, sodium, and fluoride determinations).
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PMID:Correlation of biological value of feed phosphates with their solubility in water, dilute hydrogen chloride, dilute citric acid, and neutral ammonium citrate. 147 May 90

A method has been developed for determination of arsenic in beer. Organic matter is destroyed by the dry-ashing technique, the ash is dissolved in HCl, and hydrides of arsenic are generated by addition of sodium borohydride prior to atomization in a flame-heated quartz cell and atomic absorption spectroscopy measurement. The analytical features of the method are detection limit 0.1 ng/g beer, precision 8%, and recovery 97 +/- 7%. The arsenic contents of different brands from Spain and other European countries were analyzed. In all samples, the arsenic levels found were well below maximum levels allowed in Spanish legislation (100 ng/g). The quantities of arsenic in Spanish beers do not differ from those found in foreign beers. No differences were found between bottled and canned beers, and no correlation exists between metal content and original specific gravity of the beers.
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PMID:Determination of arsenic in beer by dry ashing, hydride generation atomic absorption spectroscopy. 270 77

A digestibility experiment was carried out under conditions of reduced water supply (1/3 in comparison to the control group) and feed supply ad libitum or on maintenance level with 5 wethers of an average body weight of 43.2 kg. They were fed with alfalfa pellets with a Na supplement in the form of NaH2PO4. The water restriction increases the apparent digestibility of the nutrients (except crude fat), significantly so, however, only in the group fed ad libitum as a result of the lower digestibility in ad libitum feed intake. In the group with water restriction the absolute amount of digested nutrients is diminished, however, and in group Ib (feed supply on maintenance level) falls clearly below maintenance requirements. In a comparison of the values of apparent digestibility of the nutrients acquired by various methods (traditional method and indicator method - HCl insoluble remaining ash) the latter method is more susceptible to error. This method is, however, considerably easier in its execution. In comparison to ad libitum water supply the mean retention time of the feed increases due to restricted water supply. The decreased feed intake seems to have a bigger influence on the mean retention time of the marker than water restriction.
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PMID:[Influence of reduced water supply on the digestibility of nutrients and on some metabolic parameters in sheep]. 276 99

Mature and old B6AF1 and B6D2F1 mice were given acidified tap water or promethazine HCl (a phenothiazine with H1 receptor blocking activity), chlorpheniramine (an H1 blocker) or trifluoperazine (a phenothiazine with no H1 blocking activity) in their drinking water, and the effects of these agents on bone mineral content were assessed by intermittently measuring the 24-h whole body retention of Tc 99m methylene diphosphonate (Tc 99m MDP, an indicator of bone metabolism) and at the end of the studies by determining ash weights of femur, ilium and sacrum. It was found that 24-h retention of Tc 99m MDP was elevated in old mice as it is in old osteopenic humans, that promethazine but not chlorpheniramine or trifluoperazine inhibited bone loss in aging mice, and that there was a correlation between decrease in retention of Tc 99m MDP and decreased bone loss. These preliminary results suggest that the ability of promethazine to inhibit age-related bone loss may not be mediated through its action as an H1 blocker or as a phenothiazine. However, more agents of each type need to be tested before this point can be established.
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PMID:Age-related osteopenia in the mouse: effects of an H1 blocker, a phenothiazine, and promethazine. 287 24

Aluminum, iron, tin, zinc, calcium, magnesium, nickel, copper, chromium, cadmium, and potassium in foods can be extracted by HCl-HNO3 leaching and determined quantitatively using flame atomic absorption spectroscopy (AAS), with recoveries ranging from 90 to 110%. Thirty to 40 samples of almost any type of food sample can be analyzed routinely for 2 elements in 4-5 h. In contrast, one or 2 days are required when a wet-ash or dry-ash technique is used. Extraction consists of weighing 2-10 g samples into 125 mL Erlenmeyer flasks, adding 20 mL concentrated HCl-HNO3 (9 + 1), then heating in a 82- 93 degrees C water bath for 30 min. After cooling, samples are diluted to volume in 50 mL Nessler tubes and then filtered through No. 541 or 540 Whatman paper. The filtrate is analyzed directly by AAS.
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PMID:Determination of trace elements in foods by HCl-HNO3 leaching and flame atomic absorption spectroscopy. 302 11

Digestibility experiments were made with nearly full-grown female fattening pigs supplied with large-scale T-cannulae implanted in the distal ileum to collect the digesta in order to test 11, mainly dried and pelleted, kinds of forage plants (9 samples of lucerne cut at different times as well as green rye and whole plants of maize) as supplements to a basic diet. All samples were analysed to their content of HCl-insoluble ash as marker and their content of amino acids (AA). The calculation of the apparent precaecal and faecal crude protein (CP) digestibility and of the absorption values of the essential AA lysine, methionine, cystine, isoleucine, threonine and tryptophan as well as of glutamic acid was carried out according to the difference method. CP digestibility of the experimental diets was precaecally reduced by 5 to 20 and faecally by 3 to 13 units by supplements of at least 25% of the dry matter; for some of the AA the reduction was even bigger and dispersion between the values of the individual animals was essentially increased in comparison with the basic ration. Faecally, the precaecal differences of CP digestibility and AA absorption caused by the various alfalfa samples, are largely blurred. The CP and AA digestibility of the forage plants partly showed very big individual differences between the animals. Precaecally, a CP digestibility of more than 48% could not be ascertained. Similarly to the lysine content, precaecal lysine absorption from the kinds of roughage showed big differences. Whereas the absorption values for methionine of between 60 and 87% were relatively high, those for cystine were mostly very low, often in the negative range. This underlines that normal digestion processes are strongly influenced by high supplements of forage plants so that the constancy of the digestibility of the basic ration must be doubted under these conditions. The bacterial processes in the large intestine greatly level out the differences found precaecally between the various kinds of feedstuffs. The faecal digestibility and absorption values were considerably above those measured precaecally; methionine digestibility only was mostly considerably diminished in comparison with precaecal values due to synthesis in the large intestine. A significant positive correlation could be calculated between the lysine content of the 9 alfalfa samples (4.0 to 6.6 g/16 g N) and the apparent precaecal values of lysine absorption (2.2 to 67.6%).(ABSTRACT TRUNCATED AT 400 WORDS)
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PMID:[The apparent prececal and fecal crude protein digestibility and amino acid absorption of coarse animal feed in fattening pigs]. 320 91

Phosphorus in feeds or mineral mixes containing monobasic calcium phosphate cannot be determined accurately if the sample is subjected to the dry ash procedure described in AOAC method 7.125-7.128. HCl-insoluble calcium metaphosphates are formed at the high temperature required for ashing.
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PMID:Comments on AOAC dry ash method for digestion of mineral-mix feeds. 341 15


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