Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0205700 (ash)
 15,125 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Sixteen Chinese pigs (Meishan breed) from four litter outcome groups with initial weights ranging from 6 to 22 kg BW were used in a 28-d comparative slaughter experiment to determine the utilization of energy for maintenance and growth and the utilization of protein and amino acids (AA) by the Chinese pigs. Pigs were randomly allotted from litter outcome groups to four replicates of four pens each and to four treatments within replicates. A corn-soybean meal basal diet was formulated to provide all the nutrients except energy at twice the recommended levels for 10- to 20-kg pigs. The treatments were the basal diet fed at 3, 4, or 5% of BW. The fourth treatment was the initial slaughter group. As level of feeding increased, ADG increased linearly (P < .01) and gain:feed ratios increased quadratically (P < .06). Increasing the level of feeding had no effect on apparent digestibility coefficients of DM, N, or GE. Fecal N, urinary N, urinary urea N, and N retention increased linearly (P < .01) as feed intake increased. Level of feeding did not affect the DE, ME, or NE concentration in the diet. Metabolizable energy as a percentage of DE averaged 92.7% and was not affected by feeding level. Percentage of ether extract and DM of the empty body increased linearly (P < .01), but percentage of water decreased linearly (P < .01) with the level of feeding. Percentage of CP and ash of the empty body were not affected by the level of feeding (P > .05).(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Energy and protein metabolism of the Chinese pig. 798 36

A digestibility trial involving 20 Hampshire ram lambs and a 2-yr grazing study using 103 mature crossbred cows were conducted to determine the effects of methionine addition to a urea-grain supplement on intake and digestibility of dormant range grasses and on cow performance. In each trial, four treatment groups were supplemented with either a urea-grain control (CON), urea-grain plus methionine (MET, 3.3% DL-methionine), urea-grain plus inorganic sulfur (SUL, 3.0% sodium sulfate), or soybean meal (SBM). Supplements were designed to provide 45 and 360 g of CP.animal-1.d-1 (lambs and cows, respectively) and were balanced for ME, Ca, P, and K. Lambs had ad libitum access to mature prairie hay, whereas cows grazed dormant winter range from mid-November until mid-February. For the grazing study, forage OM intake (OMI) was determined in late November and in late January by the fecal output/indigestibility ratio technique. Controlled-release chromic oxide boluses were used as an external marker to estimate fecal output, and acid insoluble ash was used as an internal marker to predict OM digestibility (OMD). Mean daily DMI of mature prairie hay was 1,057 g/lamb and was not affected by supplementation. Apparent DM, NDF, and ADF digestibilities and N biological value did not differ (P > .10) among treatments. Nitrogen digestibility was increased (P = .06) for lambs fed the MET or SUL compared with CON.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Effect of methionine addition to a urea-grain supplement on intake and digestibility of mature, dormant grasses and performance of cows grazing winter range. 844 Jun 72

Rapid growth large frame (RL, n = 61) or average growth medium frame (AM, n = 71) biotype heifers fed to achieve either moderate (MOD, .6 kg/d) or high ADG (HI, 1.0 kg/d) were used to determine whether puberty occurs at similar body composition or metabolic status. A heifer was considered pubertal after being detected in estrus and then forming a functional corpus luteum. Live animal estimates of body composition and blood samples for assessment of metabolic status were taken at 13 +/- .2 d after estrus for all heifers. Body composition and metabolic status were assessed every 56 d from 7 mo of age until puberty in a subset of 80 heifers representing all biotype-diet combinations. At puberty, 32 of these 80 heifers were slaughtered and physical and chemical composition of the empty body were determined. High-gain diet heifers were younger, heavier, taller, and more muscular (all P < .01) at puberty than MOD heifers. Slaughter measurements paralleled live animal estimates; bodies of HI and RL heifers contained more (P < .01) carcass and noncarcass components than those of MOD and AM heifers, respectively. Carcasses of RL and HI heifers were more (P < .05) muscular and fatter than AM and MOD heifers. At puberty, HI heifers had a greater (P < .01) mass of moisture, fat, and fat-free organic matter (FFOM) than MOD, whereas RL heifers had more moisture, ash, and FFOM than AM. Percentage of fat was greater (22.1 +/- 1.0 vs 1.0 vs 19.1 +/- 1.0; P < .05) and percentage of moisture was less (55.4 +/- .6 vs 58.1 +/- .6; P < .01) in bodies of HI than in those of MOD heifers. Concentrations of blood urea nitrogen and insulin were greater (P < .05) in HI than in MOD heifers. Diet did not influence concentration of IGF-I or glucose, and metabolic markers were unaffected by biotype. No dramatic changes in body composition or metabolic signals were detected before puberty. Puberty did not occur at similar body composition or metabolic status in all heifers.
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PMID:Body composition and metabolic profiles associated with puberty in beef heifers. 858 1

Our objective was to quantify changes in supply and use of nutrients and O2 by large-frame, multicatheterized beef steers as they grew from 235 to 525 kg BW. Steers consumed 5.25 to 9.87 kg DM/d of a 62% concentrate diet that provided 126 to 217 g N/d and 1 kg ADG. Steers were assigned to three groups (eight, nine, and eight steers each) that divided the BW range into thirds. Weights at first sampling for the three groups were 236, 319, and 445 kg, respectively. Each group was sampled twice. Groups were killed after the second sampling. Tissue weights and hindquarters (HQ) contents of fat, protein, and ash were measured. Blood flow, oxygen uptake, and net uptake or release of metabolites were regressed against functions of BW.75 to assess changes during growth. Blood flow in all tissues except liver and oxygen use by all tissues decreased per unit tissue weight as BW.75 and age increased. Changes with age per unit liver weight were as follows: decreased uptake of propionate and lactate, increased uptake of alpha-amino N and glutamine, decreased production of urea and glutamate, and increased production of acetate and beta-hydroxybutyrate. Glucose and urea production per unit liver weight was constant. Changes with age per unit HQ weight were as follows: increased uptake of glucose, decreased uptake of alpha-amino N and glutamate, decreased release of lactate, and increased release of glutamine. Weight of the portal-drained viscera (PDV) increased from 91 to 97 g/kg EBW as BW increased from 236 to 522 kg; PDV fat increased from 375 to 552 g/kg PDV tissues. Liver decreased from 16 to 12 g/kg EBW. Hindquarters decreased from 286 to 266 g/kg EBW; HQ protein was 200, 197, and 200 g/kg HQ tissue for Groups 1, 2, and 3, respectively. Corresponding fat was 131, 182, and 177 g/kg HQ tissue. Changes in net flux reflect changes in nutrient partitioning and tissue deposition as steers grew and aged.
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PMID:Patterns of nutrient interchange and oxygen use among portal-drained viscera, liver, and hindquarters of beef steers from 235 to 525 kg body weight. 885 36

Ornithine transcarbamylase (OTC) deficiency, the most common and severe inborn error of the urea cycle in humans, remains without adequate treatment, and mortality rates are high. Adenoviral vectors provide an efficient system for gene delivery, but there are problems, including toxicity. Efficient promoters that reduce the amount of vector required for treatment need to be developed. We constructed two recombinant adenoviral vectors, AdexCAGhOTC and AdexSR alpha hOTC, which harbor the human OTC gene under transcriptional control of CAG (a modified chicken beta-actin promoter with CMV-IE enhancer) and SR alpha (the SV40 early promoter with the R segment and part of the US segment of the HTLV-1 LTR), respectively. Each was tested in adult spf(ash) mice, an animal model of human OTC deficiency, and in primary human hepatocytes with OTC deficiency. Spf(ash) mice have a pronounced orotic aciduria as seen in humans. A complete recovery of hepatic OTC activity with minimal tissue damage was observed in these animals following the intravenous administration of AdexCAGhOTC alone. Western blot analysis confirmed hepatic OTC expression and normalization of orotic aciduria was evident for 60 days. Enzyme activities of primary human hepatocytes infected with AdexCAGhOTC were 10-40 times higher than those with AdexSR alpha hOTC. Thus, the adenoviral vector with an efficient promoter such as CAG, can be given further consideration for possible gene therapy in humans with OTC deficiency.
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PMID:Correction of ornithine transcarbamylase deficiency in adult spf(ash) mice and in OTC-deficient human hepatocytes with recombinant adenoviruses bearing the CAG promoter. 886 Aug 34

Four Holstein steers (222 kg) with cannulas in the rumen and proximal duodenum were used in a crossover design experiment to evaluate the energy and protein value of dehydrated poultry excreta (DPE). Dietary treatments contained (DM basis) 4% alfalfa hay, 8% sudangrass hay, 49% flaked barley, 8% cane molasses, 3% yellow grease, .8% urea, 1.4% limestone, .3% mineral salt, and .4% chromic oxide, plus 25% of either tapioca pellets or DPE. The DPE contained 42% ash and 27% CP (6% true protein). Substituting DPE for tapioca decreased the DE value of the diet (P < .01). The estimated DE value of the DPE was 1.36 Mcal/kg (.34 Mcal NEm/ kg). There were no treatment effects (P > .10) on microbial N flow to the small intestine or ruminal microbial efficiency (grams of microbial N/kilogram of OM fermented). The addition of DPE increased (P < .05) the flow of non-ammonia and feed N to the small intestine. The estimated ruminal escape N value of DPE was 22%, although very little of this was true protein. Dehydrated poultry excreta did not increase (P > .10) flow of alpha-amino N to the small intestine. Ruminal degradability of uric acid was 96%. Total tract true digestibility of N in DPE was 84%. It is concluded that the NE value of DPE is markedly overestimated in current tables of feeding standards. Less than 10% of the N in DPE escapes the rumen as true protein N.
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PMID:Protein and energy value of dehydrated poultry excreta in diets for feedlot cattle. 890 99

Every 3 mo for a 2-yr period, two weaned Holstein steer calves (94.5 kg) were randomly assigned to each of four slaughter age groups (3, 6, 9, and 12 mo). Urea dilution was performed before slaughter, and urea space (US) was calculated as total volume and as a percentage of body weight (BW) and empty body weight (EBW). The relationships between US (kg, % EBW and % BW), BW, and EBW and carcass soft tissue composition (protein, fat, moisture, and ash) were studied. One- and two-pool models were fitted using the urea dilution data and the coefficients of those equations (zero time, A + B), and the intercepts of compartments A and B were used to estimate body volume. Body weight and EBW effectively predicted the amount of water, fat, and protein in the carcass soft tissue. Equations expressed in kilograms were more accurate than those expressed as percentages. Urea space overestimated body water, probably because of the fast rate of urea disappearance in plasma. Correlation coefficients between US and carcass soft tissue water (kg) based on the pooled data ranged from .74 at 6 min to .48 at 42 min after infusion. The biexponential models coefficients explained more of the variation of carcass soft tissue composition than US; correlation coefficients using volume B and the soft tissue composition (in kg) with pooled data were .78 (water), .68 (fat), .69 (ash), and .76 (protein). The relationships between A and soft tissue composition were weaker (water .59, fat .51, ash .58 and protein .59). The highest correlation coefficients were obtained when A + B was used for water, fat, ash, and protein (.83, .70, .74 and .81, respectively). Equations combining BW, EBW, and two-model coefficients (A, B, A + B) explained much of the variation of soft tissue composition. No significant benefit was found in using the urea space at various times after infusion over BW or EBW alone to estimate carcass soft tissue composition in Holstein steers.
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PMID:The use of urea dilution for estimation of carcass composition of Holstein steers at 3, 6, 9, and 12 months of age. 902 59

We hypothesized that fluoride partly acts by changing the levels of circulating calcium-regulating hormones and skeletal growth factors. The effects of oral fluoride on 24 female, Dutch-Belted, young adult rabbits were studied. The rabbits were divided into two study groups, one control and the other receiving about 16 mg fluoride/rabbit/day in their drinking water. After 6 months of fluoride dosing, all rabbits were euthanized and bone and blood samples were taken for analyses. Fluoride treatment increased serum and bone fluoride levels by over an order of magnitude (P < 0.001), but did not affect body weight or the following serum biochemical variables: urea, creatinine, phosphorus, total protein, albumin, bilirubin, SGOT, or total alkaline phosphatase. No skeletal fluorosis or osteomalacia was observed histologically, nor did fluoride affect serum PTH or Vitamin D metabolites (P > 0.4). BAP was increased 37% (P < 0.05) by fluoride; serum TRAP was increased 42% (P < 0.05); serum IGF-1 was increased 40% (P < 0.05). Fluoride increased the vertebral BV/TV by 35% (P < 0.05) and tibial ash weight by 10% (P < 0.05). However, the increases in bone mass and bone formation were not reflected in improved bone strength. Fluoride decreased bone strength by about 19% in the L5 vertebra (P < 0.01) and 25% in the femoral neck (P < 0. 05). X-ray diffraction showed altered mineral crystal thickness in fluoride-treated bones (P < 0.001), and there was a negative association between crystal width and fracture stress of the femur (P < 0.02). In conclusion, fluoride's effects on bone mass and bone turnover were not mediated by PTH. IGF-1 was increased by fluoride and was associated with increased bone turnover, but was not correlated with bone formation markers. High-dose fluoride treatment did not improve, but decreased, bone strength in rabbits, even in the absence of impaired mineralization.
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PMID:Fluoride treatment increased serum IGF-1, bone turnover, and bone mass, but not bone strength, in rabbits. 919 19

Glutamine synthetase and carbamoylphosphate synthetase I expression was examined immunohistochemically in livers of spf-ash homozygous and hemizygous mice, in which one of the urea cycle enzymes (ornithine carbamoyltransferase) is deficient and hyperammonemic disorders are obvious. In the mutant adult mouse liver, only hepatocytes lining central veins expressed glutamine synthetase. In contrast, other hepatocytes expressed carbamoylphosphate synthetase I but not glutamine synthetase. This complementary expression pattern is similar to that seen in wild-type mouse liver. In the liver of mutant young mice, which showed severe retarded growth and abnormal hair and skin development, the developmental expression pattern of both enzymes was also similar to that of the corresponding wild-type liver. However, suppression of carbamoylphosphate synthetase I expression in the pericentral hepatocytes occurred later in the mutant than in wild-type liver. These results show that high plasma concentrations of ammonium ions, which are one of the substrates for both the enzymes, do not change their complementary expression. Instead they support the idea that factor(s) associated with central veins rather than humoral factors direct pericentral hepatocytes to express glutamine synthetase and to suppress carbamoylphosphate synthetase I expression.
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PMID:Complementary expression of glutamine synthetase and carbamoylphosphate synthetase I in ornithine carbamoyltransferase-deficient mouse liver (spf-ash mouse). 945 Jun 31

Sparse fur with abnormal skin and hair (spf-ash) mice are deficient in ornithine carbamoyltransferase (OCT) activity, but their OCT protein is kinetically normal. We administered ammonium chloride to spf-ash mice, in order to analyze ammonia metabolism and to find a rationale for the therapy of OCT deficiency. Ammonia concentration in the liver of spf-ash mice increased to a level much higher than in the control. Ammonium chloride injection caused an increase in ornithine (Orn) 5 min after injection and an increase in the sum of Orn, citrulline (Cit) and arginine (Arg) for at least 15 min in the liver of control mice, but no increase in Orn, Cit and Arg in the liver of spf-ash mice. Treatment of spf-ash mice with Arg 5-20 min prior to the injection of ammonium chloride kept the hepatic ammonia concentration at a level comparable to that without the load. A significant reciprocal relationship between ammonia and Orn concentrations in the liver of spf-ash mice 5 min after an ammonium chloride load with or without Arg strongly suggests that ammonia disposal is dependent on the supply of Orn. In spf-ash mice loaded with tryptone as a nitrogen source, Arg supplementation showed a dramatic decrease in urinary orotic acid excretion in a dose-dependent manner. Similar effects were observed with Cit and Orn at the same dose, and a long-lasting effect with an ornithine aminotransferase inactivator, 5-(fluoromethyl)ornithine, at a much lower dose. The rate of urea formation in liver perfused with ammonium chloride was lower in spf-ash mice than in controls, but with the addition of Orn to the medium it increased to a similar level in control and spf-ash mice. These results indicate that OCT is not saturated with Orn in vivo under physiological conditions and that the administration or enrichment of the urea cycle intermediate amino acids enhances the OCT reaction so that the ammonia metabolism of OCT-deficient spf-ash mice is at least partially normalized.
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PMID:Aberrations of ammonia metabolism in ornithine carbamoyltransferase-deficient spf-ash mice and their prevention by treatment with urea cycle intermediate amino acids and an ornithine aminotransferase inactivator. 1052 24


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