Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: UMLS:C0178874 (
tumor progression
)
40,807
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Nuclear ubiquitous casein and cyclin-dependent kinases substrate
(
NUCKS
) is 27 kDa chromosomal protein of unknown function. Its amino acid composition as well as structure of its DNA binding domain resembles that of high-mobility group A, HMGA proteins. HMGA proteins are associated with various malignancies. Since changes in expression of HMGA are considered as marker of
tumor progression
, it is possible that similar changes in expression of
NUCKS
could be useful tool in diagnosis and prognosis of breast cancer. For identification and analysis of
NUCKS
we used proteomic and histochemical methods. Analysis of patient-matched samples of normal and breast cancer by mass spectrometry revealed elevated levels of
NUCKS
in protein extracts from ductal breast cancers. We elicited specific antibodies against
NUCKS
and used them for immunohistochemistry in invasive ductal carcinoma of breast. We found high expression of
NUCKS
in 84.3% of cancer cells. We suggest that such overexpression of
NUCKS
can play significant role in breast cancer biology.
...
PMID:Immunohistochemical and proteomic evaluation of nuclear ubiquitous casein and cyclin-dependent kinases substrate in invasive ductal carcinoma of the breast. 2006 58
Nuclear ubiquitous casein and cyclin-dependent kinases substrate
(
NUCKS
) is a chromosomal protein of unknown function. Its amino acid composition and structure of its DNA binding domain resemble those of high mobility group A (HMGA) proteins which are associated with various malignancies. Since changes in expression of HMGA are considered as a marker of
tumor progression
, it is possible that similar changes in expression of
NUCKS
could be a useful tool in diagnosis of malignant skin tumors. To investigate this assumption we used specific antibodies against
NUCKS
for immunohistochemistry of squamous (SCC) and basal cell carcinoma (BCC) as well as keratoacanthoma (KA). We found high expression of
NUCKS
in nuclei of SCC and BCC cells which exceeded expression of the well-known proliferation marker Ki67. Expression of
NUCKS
in benign KA was much below that of malignant tumors. With the present study and based on our previous experience we would like to suggest the NUCKS protein as a novel proliferation marker for immunohistochemical evaluation of formalin-fixed and paraffin-embedded skin tumor specimens. We would like to emphasize that
NUCKS
abundance in malignant skin tumors is higher than that of the well-known proliferation marker Ki67, thus allowing more precise assessment of tumor proliferation potential.
...
PMID:The comparison of nuclear ubiquitous casein and cyclin-dependent kinases substrate (NUCKS) with Ki67 proliferation marker expression in common skin tumors. 2511 9
