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Query: UMLS:C0162871 (abdominal aortic aneurysm)
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A comparative proteomics analysis was performed to identify the molecular response of a rice cultivar (Oryza sative cv. 'IRRI71331') with high phosphorous (P) uptake efficiency to low P stress. The hydroponically grown rice plants were provided with two levels of P (0.5 mg x L(-1) and 10 mg x L(-1)) supplied in quarter strength Kimura solution, and the root total proteins extracted on the 3rd and 6th day of treatments were separated by two-dimensional gel electrophoresis (2-DE). Comparing with the control (10 mg x L(-1) of P), a total of 29 protein spots under low P stress (0.5 mg x L(-1)) showed differences in their relative abundance, among which, 17 were higher, 11 were lower, and 1 was novel on the 3rd day, and 8 were induced, 19 were suppressed, 1 was disappeared, and 1 had no obvious change on the 6th day. Ten differentially expressed protein spots were identified by MALDI-TOF/MS, and searched in protein databases. According to the putative functions, the identified proteins were classified into four groups, i.e., signal transduction (glycine-rich RNA-binding protein, phosphate starvation response regulator-like), gene expression (putative pre-mRNA splicing factor, putative AAA-metalloprotease), metabolism (adenylosuccinate lyase, serpin, S-adenosylmethionine synthetase, MYB transcription factor-like protein), and ion transport (cation-transporting ATPase, sarcoplasmic reticulum protein). The identified proteins were involved in various physiological responses to enhance stress resistance, such as signal recognition and transduction, RNA cleavage, degradation of denatured protein, and ion transportation and cellular ion balance. The serine protease inhibitor and S-adenosylmethionine synthetase and the MYB transcription factor-like protein, which were the key proteins associated with P deficiency--tolerance of other species, were affected by the same stress for rice. The results indicated that the tolerance to low P stress was controlled by a complex signal transduction and metabolism regulation network in rice root system.
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PMID:[Differential protein analysis on the root response of rice with high phosphorous uptake efficiency to low phosphorous stress]. 2144 14

Somatic embryos are comparable to their zygotic counterparts for morphological traits but are derived from somatic cells through various metabolic regulations, collectively referred as somatic embryogenesis (SE). It has been well exploited for germplasm conservation, genetic engineering, mutation breeding, for artificial seed technology and as a tool for mass multiplication. Though somatic embryo development is an important area of interest in growth, and developmental studies, the underlying molecular mechanism remains unclear. Therefore, understanding the molecular basis behind somatic embryo development can provide insight into the signaling pathways integrating this process. Proteomic analysis of somatic embryo development in cv. Grand Naine (AAA) was carried out to identify the differentially expressed protein during somatic embryo development stages, using two dimensional gel electrophoresis together with mass spectrometry. In total, 25 protein spots were differentially expressed during sequential developmental stages of somatic embryos. Among these, three proteins were uniquely present in 30 days globular stage and six proteins in 60 days old mature somatic embryo. Functional annotation of identified spots showed that major proteins are involved in growth and developmental process (17%) followed by defense response (12%) and signal transportation events (12%). In the early stage, cell division and growth related proteins are involved in the induction of somatic embryos whereas in the late developmental stage, cell wall associated proteins along with stress related proteins played a defensive role against dehydration and osmotic stress and resulted in the maturation of somatic embryo. The identified stage specific proteins are valuable indicators and genetic markers for screening and for media manipulation to improve SE efficiency in recalcitrant crops and varieties.
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PMID:Proteomic analysis of somatic embryo development in Musa spp. cv. Grand Naine (AAA). 3216 9