Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0162871 (abdominal aortic aneurysm)
 8,664 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Many retroviruses express gag-pol or gag-pro-pol polypeptides by coupling their translation from overlapping reading frames with -1 ribosomal frameshifts. Here, we show that the well-known ribosomal frameshift signals found in retroviral mRNA will provoke Escherichia coli ribosomes to shift frame in the same manner as their eukaryotic counterparts. Ribosomes of E. coli respond in vivo to both the tandem slippery codons present at the retroviral frameshift site and the 3' flanking sequence. Slight alteration of the mouse mammary tumor virus gag-pro frameshift site from A-AAA-AAC to A-AAA-AAG boosts the level of frameshifting in E. coli to over 50%. This suggests that A-AAA-AAG, and its slippery relatives, may be utilized by E. coli genes as sites of high-level ribosomal frameshifting. This observed conservation of response to retroviral frameshift signals affords new avenues to dissect the mechanism of ribosomal frameshifting evoked by these mRNA sequences.
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PMID:E. coli ribosomes re-phase on retroviral frameshift signals at rates ranging from 2 to 50 percent. 256 19