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Target Concepts:
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Query: UMLS:C0162871 (
abdominal aortic aneurysm
)
8,664
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We obtained two novel W chromosome-linked chick genes by the use of female-male subtraction macroarrays, one of which, 2d-2F9, (recorded as AB188527 in DDBJ) did not have sufficient length (776 bp) to reveal its real form or characteristics. Hence, we obtained full-length Z-linked and W-linked 2d-2F9 genes of 2596 bp and 2589 bp respectively by the oligo-capping and
RACE
methods. Sequence analysis of these genes not only revealed that there is a counterpart of the W-linked 2d-2F9 gene on the Z chromosome, but also that there is a low homologous area at 5'-UTR between the W- and Z-kinked genes. Using this information, we designed a set of primers to identify sex and to select clones having the Z and W-linked gene (named 2d-2F9-Z and 2d-2F9-W), and also prepared two sets of primers for RT-PCR. These genes were found to be expressed constitutively and ubiquitously from the early embryo to the hatched chick, and they were assigned to the
AAA
ATP-superfamily.
...
PMID:Sequence analysis of full-length cDNA of sex chromosome-linked novel gene 2d-2F9 in Gallus gallus. 1728 46
Mitochondria and autophagy play important roles in the networks that regulate plant leaf senescence and cell death. However, the molecular mechanisms underlying the interactions between mitochondrial signaling and autophagy are currently not well understood. This study characterized the function of the Arabidopsis (
Arabidopsis thaliana
) mitochondrial
AAA
-protease gene
FtSH4
in regulating autophagy and senescence, finding that FtSH4 mediates WRKY-dependent salicylic acid (SA) accumulation and signaling. Knockout of
FtSH4
in the
ftsh4-4
mutant resulted in severe leaf senescence, cell death, and high autophagy levels. The level of SA increased dramatically in the
ftsh4-4
mutant. Expression of
nahG
in the
ftsh4-4
mutant led to decreased SA levels and suppressed the leaf senescence and cell death phenotypes. The transcript levels of several SA synthesis and signaling genes, including
SALICYLIC ACID
INDUCTION DEFICIENT2
(
SID2
),
NON-
RACE
-SPECIFIC DISEASE RESISTANCE1
(
NDR1
), and
NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1
(
NPR1
), increased significantly in the
ftsh4-4
mutants compared with the wild type. Loss of function of
SID2
,
NDR1
, or
NPR1
in the
ftsh4-4
mutant reversed the
ftsh4-4
senescence and autophagy phenotypes. Furthermore,
ftsh4-4
mutants had elevated levels of transcripts of several
WRKY
genes, including
WRKY40
,
WRKY46
,
WRKY51
,
WRKY60
,
WRKY63
, and
WRKY75
; all of these WRKY proteins can bind to the promoter of
SID2
Loss of function of
WRKY75
in the
ftsh4-4
mutants decreased the levels of SA and reversed the senescence phenotype. Taken together, these results suggest that the mitochondrial ATP-dependent protease FtSH4 may regulate the expression of
WRKY
genes by modifying the level of reactive oxygen species and the WRKY transcription factors that control SA synthesis and signaling in autophagy and senescence.
...
PMID:The Arabidopsis Mitochondrial Protease FtSH4 Is Involved in Leaf Senescence via Regulation of WRKY-Dependent Salicylic Acid Accumulation and Signaling. 2825 67
