Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0162871 (
abdominal aortic aneurysm
)
8,664
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
E. coli 1-phosphate
mannitol dehydrogenase
gene (mtl-D) was cloned using PCR method. Sequence analysis showed that the gene was the same as the published one except that codon CAT at position 416 was replaced by
AAA
and resulted in a Lys residue instead of His. This gene (mtl-D) was inserted in a binary vector and transformed into populas via agrobacteria. Several transgenic plants grow very well in 0.6% NaCl while controls can not survive even in 0.4% NaCl. PCR analysis and Northern blotting indicated that foreign gene was integrated into the genome of transgenic plant and transcribed successfully.
...
PMID:[Cloning of E. coli mtl-D gene and its expression in transgenic Balizhuangyang (Populus)]. 1097 89
