Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0162871 (abdominal aortic aneurysm)
 8,664 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The photochemical behaviour of three relevant metabolites of the analgesic and antipyretic drug dipyrone, 4-methylaminoantipyrine (4-MAA), 4-formylaminoantipyrine (4-FAA) and 4-acetylaminoantipyrine (4-AAA), was evaluated under simulated solar irradiation (Suntest system). For 4-MAA, different aqueous solutions (synthetic seawater, freshwater and Milli-Q water) as well as different operational conditions were compared. According to the experimental results, 4-MAA resulted as being an easily degraded molecule by direct photolysis, with half-life times (t1/2) ranging from 0.12 to 0.58 h, depending on the irradiation conditions. Faster degradation was observed in synthetic waters, suggesting that the photolysis was influenced by the salt composition of the waters. However, no effect on the degradation rate was observed by the presence of natural photosensitizers (dissolved organic matter, nitrate ions). 4-FAA and 4-AAA showed slower photodegradation kinetics, with t1/2 of 24 and 28 h, respectively. A study of photoproduct identification was carried out by gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-time-of-flight mass spectrometry (LC-TOF-MS) (ESI positive mode), which allowed us to propose a tentative photodegradation pathway for 4-MAA and the identification of persistent by-products in all the cases. Finally, the application of an acute toxicity test (Daphnia magna) showed an increase in toxicity during the photolytic process, a consequence of the formation of toxic photoproducts.
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PMID:Photodegradation study of three dipyrone metabolites in various water systems: identification and toxicity of their photodegradation products. 1829 72

One of the possibilities to study abdominal aortic aneurysm (AAA) biomarkers is to use -aneurysm biopsies of dilated arteries or intraluminal thrombus (ILT) for explant cultures. Those metabolites secreted into the culture medium are concentrated in comparison to plasma and free from other interferences that can appear in it, allowing an easier harvesting. Liquid chromatography coupled to quadrupole time-of-flight mass spectrometry detector (LC-QTOF-MS) with an electrospray (ESI) interface has a broad applicability to detect metabolites of all classes with high sensitivity and mass accuracy. Therefore, an LC-QTOF-MS-based metabolomics approach was chosen to select and identify metabolites that could be useful as diagnostic/prognostic markers of AAA. This chapter describes the methodology for the differential analysis of AAA metabolites in secretomes. It gives experimental details on basic steps such as sample preparation, protein precipitation, and analysis of the samples by LC-MS. A description for quality control of the methodology, raw data processing, as well as selection and identification of differentiating -metabolites are also presented in this chapter.
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PMID:Metabolites secreted by human atherothrombotic aneurysm. 2358 88