Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
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Gene/Protein
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Target Concepts:
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Query: UMLS:C0162473 (
Frey
)
2,599
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
UDP-galactose 4-epimerase from Escherichia coli contains tightly bound
NAD+
, which participates in catalyzing the interconversion of UDP-galactose and UDP-glucose through its redox properties. The purified enzyme is a dimer of identical subunits that consists of a mixture of catalytically active subunits designated E.
NAD+
and inactive, abortive complexes designated E.NADH.uridine nucleotide, in which the uridine nucleotide may be UDP-glucose, UDP-galactose, or UDP [Vanhooke, J. L., &
Frey
, P. A. (1994) J. Biol. Chem. 269, 31496-31404]. The abortive complexes are transformed into active E.
NAD+
by denaturation of the purified enzyme at 4 degrees C in 6 M guanidine hydrochloride buffered at pH 7.0 in the presence of 0.126 mM
NAD+
for 3 h, followed by dilution of guanidine hydrochloride to 0.18 M and of
NAD+
to 0.076 mM for 2 h. The renatured enzyme is fully active and contains negligible amounts of NADH and uridine nucleotides. The extinction coefficent of the epimerase at 280 nm is 1.81 +/- 0.15 mL mg-1 cm-1 (epsilon 280 = 137 +/- 11 mM-1 cm-1), as determined by quantitative amino acid analysis and spectrophotometric measurements. This value allows the value of the extinction coefficient for the reduced enzyme (E.NADH)to be calculated as epsilon 344 = 5.7 mM-1 cm-1. On the basis of the new value of epsilon 280, analytical measurements of the nAD+ content of epimerase show that there are two molecules of
NAD+
per dimer, which confirms conclusions from X-ray crystallography and revises the earlier bioanalytical determinations. The ultraviolet/visible absorption spectrum of E.
NAD+
from denaturation-renaturation experiments reveals the presence of a broad absorption band extending from 300 nm to beyond 360 nm that cannot be attributed to NADH and appears to be a charge-transfer band. This band is partially bleached by UMP and almost totally abolished by UDP, indicating that the interactions leading to the charge-transfer band are altered by the uridine nucleotide-induced conformational change in this enzyme. This conformational change is associated with control of the chemical reactivity of
NAD+
in the reaction mechanism.
...
PMID:UDP-galactose 4-epimerase: NAD+ content and a charge-transfer band associated with the substrate-induced conformational transition. 865 44
