Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0162473 (Frey)
 2,599 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spinach-leaf ribulose-5-phosphate kinase catalyzes the reaction of (Rp)-[beta, gamma-18O, gamma-18O]adenosine 5'-(3-thiotriphosphate) with ribulose 5-phosphate to form ribulose 1-[18O]phosphorothioate 5-phosphate. This product is incubated with CO2, Mg2+, and ribulose-bisphosphate carboxylase to form the [18O]phosphorothioate of D-glycerate. Reduction of this material using phosphoglycerate kinase/ATP, glyceraldehyde-3-phosphate dehydrogenase/NADH, triose-phosphate isomerase, and glycerol-phosphate dehydrogenase/NADH produces glycerol 3-[18O]phosphorothioate, which is subjected to ring closure using diethylphosphorochloridate. This in-line reaction produces a diastereoisomeric mixture of glycerol 2,3-cyclic phosphorothioates. 31P NMR spectroscopy was used to analyze the 18O content of the products. The anti-diastereoisomer, which is the major isomer formed and corresponds to the downfield 31P NMR signal (Pliura, D.H., Schomburg, D., Richard, J.P., Frey, P.A., and Knowles, J.R. (1980) Biochemistry 19, 325-329), retains the 18O label. This observation indicates that the ribulose-5-phosphate kinase reaction proceeds with inversion of configuration at phosphorus. The reaction is, therefore, unlikely to involve the participation of a covalent phosphoryl-enzyme intermediate.
 ...
PMID:The stereochemical course of the ribulose-5-phosphate kinase-catalyzed reaction. 649 Jun 43

A novel in vitro bladder preparation was used to examine effect of various stimuli (stretch, von Frey hair compression, stroking of receptive fields, applications of chemical stimuli to the mucosa) on electrophysiological recordings from guinea pig bladder afferents in vitro. Several functionally distinct classes of bladder sensory neurons were distinguished. These include stretch-sensitive afferents-muscle mechanoreceptors which behaved as "in-series tension receptors" and tension-mucosal mechanoreceptors, which could be activated by stretch, mucosal stroking with light von Frey hair (0.1-2 mN) and by hypertonic solutions (1 M mannitol and 490-850 mM NaCl) applied locally to their receptive fields in the mucosa. In addition, we have recorded stretch-insensitive afferents-mucosal mechanoreceptors and chemoreceptors. The non-selective P2X/P2Y purinoreceptor antagonist, PPADS (30 microM) did not affect stretch-induced firing by low threshold muscle mechanoreceptors but significantly inhibited alpha,beta-methylene ATP (30 microM)-induced contractions and associated afferent firing. Transduction by low threshold stretch-sensitive muscle mechanoreceptors does not appear to involve exocytotic synaptic transmission since it occurs in Ca2+-free (with 1 mM EDTA and 6 mM Mg2+) Krebs solution. The data suggest that the endogenous transmitter ATP is not involved in mechanotransduction by this specific class of low threshold muscle mechanoreceptors in the guinea pig bladder; rather they appear to transduce mechanical stimuli directly, possibly via stretch-activated ion channels. Mechanisms of activation of other classes of mechanoreceptors and chemoreceptors remain to be established.
 ...
PMID:Major classes of sensory neurons to the urinary bladder. 1658 9