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Query: UMLS:C0162473 (
Frey
)
2,599
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Given the role of Ca
v
3.2 isoform among T-type Ca
2+
channels (T-channels) in somatic and visceral nociceptive processing, we analyzed the contribution of Ca
v
3.2 to butyrate-induced colonic pain and nociceptor hypersensitivity in mice, to evaluate whether Ca
v
3.2 could serve as a target for treatment of visceral pain in irritable bowel syndrome (IBS) patients. Mice of ddY strain, and wild-type and Ca
v
3.2-knockout mice of a C57BL/6J background received intracolonic administration of butyrate twice a day for 3 days. Referred hyperalgesia in the lower abdomen was assessed by von
Frey
test, and colonic hypersensitivity to distension by a volume load or chemicals was evaluated by counting nociceptive behaviors. Spinal phosphorylated ERK was detected by immunohistochemistry. Ca
v
3.2 knockdown was accomplished by intrathecal injection of antisense oligodeoxynucleotides.
Butyrate
treatment caused referred hyperalgesia and colonic hypersensitivity to distension in ddY mice, which was abolished by T-channel blockers and/or Ca
v
3.2 knockdown.
Butyrate
also increased the number of spinal phosphorylated ERK-positive neurons following colonic distension in the anesthetized ddY mice. The butyrate-treated ddY mice also exhibited T-channel-dependent colonic hypersensitivity to intracolonic Na
2
S, known to enhance Ca
v
3.2 activity, and TRPV1, TRPA1 or proteinase-activated receptor 2 (PAR2) agonists. Wild-type, but not Ca
v
3.2-knockout, mice of a C57BL/6J background, after treated with butyrate, mimicked the T-channel-dependent referred hyperalgesia and colonic hypersensitivity in butyrate-treated ddY mice. Our study provides definitive evidence for an essential role of Ca
v
3.2 in the butyrate-induced colonic pain and nociceptor hypersensitivity, which might serve as a target for treatment of visceral pain in IBS patients.
...
PMID:Essential role of Ca
v
3.2 T-type calcium channels in butyrate-induced colonic pain and nociceptor hypersensitivity in mice. 3294 97
