Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0162473 (
Frey
)
2,599
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Filamentous fungi and bacteria form mixed-species biofilms in nature and diverse clinical contexts (
Frey
-Klett
et al.
, 2011; Peleg
et al.
, 2010). The interactions between fungi and bacteria, often mediated by secreted metabolites, have important ramifications for the biology of the interacting partners (
Frey
-Klett
et al.
, 2011). This is particularly true for the bacterium
Pseudomonas aeruginosa
(
P. aeruginosa
) and the fungus
Aspergillus fumigatus
(
A. fumigatus
) which often reside in the same niche such as lungs of
cystic fibrosis
(CF) patients. Some studies have reported that co-infection with
P. aeruginosa
and
A. fumigatus
could lead to a decrease in lung function relative to their respective single species infection (Amin
et al.
, 2010; Peleg
et al.
, 2010). Metabolite extraction and analysis allow for the characterization of specific microbial metabolites in the polymicrobial biofilm. This protocol describes how to prepare the
Pseudomonas-Aspergillus
co-culture biofilm on solid medium in preparation for metabolite extraction.
...
PMID:Establishing a Biofilm Co-culture of
Pseudomonas
and
Aspergillus
for Metabolite Extraction. 2910 89
