Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0155339 (Brown)
 12,436 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

We examined whether Brown Norway rat plasma (BN/May Pfd f) contains alpha 1-cysteine proteinase inhibitor (alpha 1-CPI), also called major acute phase alpha 1-protein or T-kininogen. T-kininogen is a low molecular weight kininogen from which kinin can be released by trypsin but not by kallikreins. The BN plasma reacted with rabbit anti-alpha 1-CPI gamma globulins. Purified alpha 1-CPI released a kinin-like activity with trypsin and with homogenate of salivary glands, as Brown Norway rat plasma did. High concentration of added rat urine induced a small release (10%) of kinin from alpha 1-CPI. Preincubation of Brown Norway rat plasma with rabbit anti-rat alpha 1-CPI gamma-globulins nearly suppressed the kinin-forming substrate of trypsin in this plasma. These results indicated that plasma of our Brown Norway rats contains only alpha 1-CPI as kinin-forming substrate. This plasma contains low amount of alpha 2-macroglobulin, while its content in orosomucoid and haptoglobin was a little larger than that of Wistar rat plasma.
 ...
PMID:Acute phase plasma proteins in kininogen-deficient Brown Norway rats. 243 May 37

A direct radioimmunoassay (RIA) for rat high molecular weight kininogen (HMW Kg) was developed that enabled us to detect 71 fmol/ml of HMW Kg. The antibodies did not crossreact in the RIA with up to 5 nmol of purified rat alpha 1 cysteine proteinase inhibitor (T-kininogen). When various quantities of pure HMW Kg were either quantified by the RIA or by the measurement of kinin contents determined after trypsin hydrolysis, identical values were obtained by both methods. This RIA allowed the measurement of HMW Kg in 0.015 to 1 microliter of rat plasma. HMW Kg levels in plasma of Wistar rats and in Brown Norway rats of the Orlean Strain (BN/Orl) were 1.52 +/- 0.05 (n = 6) and 2.050 +/- 0.015 (n = 8) nmol/ml respectively. In the Brown Norway rats of the Katholiek strain (BN/Kat) that are considered to be deficient in HMW Kg, immunoreactive HMW Kg levels were less than 2% of those of the BN/Orl rats. These results confirm that the BN/Kat animals have a molecular defect in HMW Kg.
 ...
PMID:Direct radioimmunoassay for rat high molecular weight kininogen. Measurement of immunoreactive high molecular weight kininogen in normal and kininogen deficient plasma. 348 Dec 12