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Query: UMLS:C0155339 (Brown)
 12,436 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The nucleolar organizer-specific staining procedure, ammoniacal silver (Ag-AS), has been used to study the distribution and size of the nucleolar organizer regions (NORs) in chromosomes of the frog Rana blairi (Mecham, Littlejohn, Oldham, Brown and Brown). The somatic metaphase karyotype of this frog is similar to that of other frogs of the Rana pipiens species complex, numerically (2n=26) and morphologically. Secondary constrictions are detectable in untreated Giemsa-stained metaphase preparations as achromatic gaps in the long arms of a pair of submetacentric chromosomes (no. 10). These constrictions are the only regions which are deeply stained with the Ag-AS method and are thus identified as the nucleolar organizer regions (Ag-NORs). In each of the three individuals, the Ag-NORs as visualized on the homologues are of unequal length.
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PMID:Dimorphic nucleolar organizer regions in the frog Rana blairi. 6 82

A 4-year-old Santa Gertrudis cow had epistaxis and loud nasal sounds associated with nasal granulomas. Multiple cutaneous granulomatous nodules were on the ears and single ones on the tail, vulva and thigh. Helminthosporium sp. was isolated from cultured lesions; two isolates from the ear and nasal mucosa were identified as H. speciferum. Tissue sections stained with hematoxylin and eosin contained rounded forms ranging from 5X5 micronM to 12X19 micronM; an indistinct hyaline wall surrounded granules and vacuoles of varying size. Occasional transverse septa were present. Periodic acid-Schiff, Gomori's Methenamine Silver and Gridley's Fungus stain treated sections contained numerous smaller pleomorphic buds and a few branching septate hyphae attached to the rounded forms. Brown pigmentation of the wall was not a consistent finding.
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PMID:Helminthosporium speciferum as the cause of dermal and nasal maduromycosis in a cow. 87 Feb 86

DNA containing the reiterated genes for tRNA1met has been partially purified from Xenopus laevis by centrifugation in actinomycin C1-CsCl and Ag+-Cs2SO4 gradients. These gradients separate the tRNA1met genes from those coding for tRNA2met and tRNAval, thus confirming our earlier suggestion that these genes are not intermingled with each other (Clarkson, Birnstiel, and Purdom, 1973a). The gradients also demonstrate the existence of a minor 5S DNA fraction which appears to differ from that previously isolated by Brown, Wensink, and Jordon (1971). When the enriched tDNA1met is digested to completion with either of the restriction endoncucleases EcoRl or Hpa l, the tRNA1met genes are predominantly found within DNA fragments that are about 3100 base pairs long. A partial digestion with EcoRl shows that these fragments arise from the regular spacing of the enzyme restriction sites. The 3100 base pair EcoRl fragments are cleaved by Hpa l into fragments to two size classes, one of which is about 2200 base pairs long and contains the tRNA1met genes. The shorter fragments are about 700 base pairs long, and they appear to contain genes coding for at least one other kind of tRNA species. X. laevis tDNA1met thus comprises tandemly repeated DNA whose component parts show little if any length heterogeneity.
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PMID:Isolation and some properties of DNA coding for tRNA1met from Xenopus laevis. 98 75

After injection of Plasmodium berghei sporozoites into Norway-Brown rats, we were able to localize these sporozoites and the early hepatic trophozoites developing from them in histological sections of the liver stained with a sensitive immunogold-silver procedure. Sporozoites invading hepatocytes released substantial quantities of circumsporozoite protein into the hepatocyte cytoplasm. This intrahepatic cytoplasmic distribution reached a maximal level at about 4 h post-sporozoite injection. As the hepatic parasites continued to differentiate, circumsporozoite protein became undetectable within the cytoplasm of the hepatocytes and became localized around the periphery of each parasite. There was generalized cellular inflammation within the liver of the host, which first became evident at around 4 h post-sporozoite injection and progressed to the formation of well-defined granulomas by 24 h. Such histopathological changes were not seen in rats injected with killed sporozoites, indicating that the cellular inflammation was induced by viable, infective sporozoites. We did not observe cellular infiltration specifically associated with any of the developing hepatic stages that we observed, even up to 28 h post-sporozoite inoculation. These results indicate that viable sporozoites induced rapid and generalized hepatic inflammation in host rats. However, sporozoites that successfully invaded hepatocytes and then proceeded to develop further did not appear to be the target of inflammatory cells until a period beginning at around 40 h post-sporozoite inoculation.
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PMID:Early hepatic stages of Plasmodium berghei: release of circumsporozoite protein and host cellular inflammatory response. 172 89

Cat-scratch disease is a benign inoculative lymphoreticulosis, related to the presence of a polymorph bacillus, Warthin-Stary silver stained, Gram negative as assessed by Brown-Hopp staining. It is found in the capillary walls and in macrophages bordering the lymph node sinusoids at the site of inoculation, in regional subacute adenopathy before softening, in internal organs and blood cultures of systemic infections, occurring more often in immuno-compromised patients. These bacteria have been demonstrated in subcutaneous vascular nodules, near to histiocytoid hemangioma in AIDS patients; these lesions are very similar to early stage Kaposi's sarcomas. This bacteria is provisionally listed as G 1492 by the Center for Disease Control.
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PMID:Cat-scratch disease bacteria. 210 81

After oral surgery, a 32-year-old man developed a brain abscess. Actinomycosis was suspected due to history, clinical findings, response to penicillin therapy, and demonstration of "sulfur granules" in the surgical specimen, but anaerobic cultures were negative for Actinomyces. Aerobic cultures yielded Streptococcus sanguis and Pseudomonas cepacia. Coccoid organisms demonstrated histologically reacted positively with periodic acid-Schiff, Gomori's methenamine silver, and Brown and Brenn stains, were Ziehl-Neelsen-negative, and did not include branching filaments. Fluorescent antibody assay for Actinomyces israelii was also negative. Electron microscopy revealed cell wall morphology and pattern of cell division characteristic of gram-positive cocci. These findings led to a final diagnosis of botromycosis due to S. sanguis. This third report of cerebral botryomycosis emphasizes the differential diagnosis with actinomycosis, the association with intermittently treated jaw disease, and identification of the causative agent by histologic, immunologic, and electron microscopic methods.
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PMID:Cerebral botryomycosis: case study. 238 2

A retrospective study of lymph node biopsy specimens from nine patients with the clinical findings and histologic features of cat scratch disease was undertaken to determine whether the recent report by Wear et al. that pleomorphic bacteria are present in the lymph nodes of cat scratch disease could be confirmed. In seven of our nine cases, pleomorphic bacteria were demonstrated with the Warthin-Starry (WS) silver stain. These were gram-negative with the Brown-Hopps tissue Gram stain and were almost at the limit of microscopic resolution. Lymph node specimens from 13 additional patients with nonspecific lymphadenitis who had neither clinical nor histologic findings of cat scratch disease were studied similarly; in none of these were bacteria demonstrated with the WS silver stain. After examining the distribution of the organisms and the related morphologic features in cat scratch disease, we conclude that demonstration of pleomorphic, gram-negative, WS-positive bacteria in the appropriate clinical and histologic setting can firmly establish the diagnosis of cat scratch disease.
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PMID:Cat scratch disease. Identification of bacteria in seven cases of lymphadenitis. 242 62

Campylobacter pylori has been implicated in the pathogenesis of peptide ulcer disease. The rapid identification of this organism may depend upon histologic diagnosis, because culture methods are complex and require a minimum of seven days in order to identify a negative specimen. The purpose of this study was to determine which stain used to identify this organism was the most cost-effective and easiest to perform and interpret on a routine basis. Sixty-one consecutive gastric antral biopsies were stained with hematoxylin and eosin, Giemsa, Brown-Brenn, and Warthin-Starry, with 23 of the cases stained by Brown-Hopps. Of the stains tested, the Wright-Giemsa was the easiest to perform. The organisms on the Wright-Giemsa showed a smooth, uniform purple color, whereas the Warthin-Starry gave the organism a granular appearance that at times could be confused for silver precipitate. Both the Wright-Giemsa and Brown-Hopps stain had the highest degree of identification of the organism (defined by percent positivity). The routine use of the Wright-Giemsa stain for identification of C. pylori in antral biopsies is recommended.
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PMID:Evaluation of staining methods for identifying Campylobacter pylori. 245 56

This study was performed to observe the role of Pneumocystis carinii as an etiologic agent of interstitial pneumonia in immunocompromised hosts. Total 90 male Sprague-Dawley rats, approximately 150-180 g, were used. Fifteen of them were used as control group and remaining 75 (5 groups) were as immunosuppression groups; group 1 received prednisolone (25 mg/kg twice weekly) only; group 2 prednisolone and tetracycline (75 mk/kg/day); group 3 prednisolone, tetracycline and trimethoprim-sulfamethoxazole (50-250 mg/kg/day); group 4 prednisolone and trimethoprimsulfamethoxazole; and group 5 prednisolone and griseofulvin (300 mg/kg/day) until death. The survival days of each group rat were calculated, and upon death their lungs were removed immediately and then stamp smears were prepared and stained by Giemsa or toluidine blue O. For histopathologic observation, lungs were fixed in 10% formalin, cut into sections and stained with Gomori's methenamine silver, hematoxylin-eosin, and Brown & Brenn stain. The results obtained were as follows: 1. The mean survival time of each group rat was 19.3 +/- 5.2 days (group 1), 41.1 +/- 14.0 days (group 2), 50.5 +/- 18.4 days (group 3), 43.0 +/- 22.9 days (group 4) or 21.8 +/- 5.1 days (group 5). Significant differences were noted between group 1 and group 2(p less than 0.01), group 1 and group 3 (p less than 0.01), and group 1 and group 4 (p less than 0.01), which represented bacterial infections were most fatal in immunocompromised rats.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:[An experimental study on prednisolone-induced interstitial pneumonia caused by Pneumocystis carinii]. 248 28

Autoimmune tubulointerstitial nephritis (TIN) was induced in Lewis (LEW) rats by immunization with homologous Brown-Norway (BN) rat renal basement membrane (RBM), complete Freund's adjuvant and Bordetella pertussis vaccine. The BN strain has a tubular basement membrane (TBM) antigen (Ag+) detectable by immunofluorescence which is lacking in unmodified LEW rat TBM. Development of TIN in LEW rats correlated with TBM Ag+ immunogens from homologous and heterologous RBM preparations. By day 14 after immunization TIN developed characterized by elevated serum creatinine levels and by tubular destruction with focal, circumscribed lesions containing epithelioid cells, giant cells and mononuclear cell infiltrates. Approximately 60% of the mononuclear cells bore T cell antigens with most cells expressing Ia markers. Immunofluorescence and elution studies revealed no selective IgG fixation to TBM at day 14 despite high titers of circulating alloantibody reactive with the immunizing TBM. Intravenous transfer of LNC and/or splenic cells (3.5 to 7 X 10(8)) to naive LEW rats resulted in less severe but histologically identical TIN in seven days with T cell subpopulations similar to those seen in the active model. This model strongly suggests an initiating role for cell-mediated immunity in TIN in the rat and may provide a parallel to human TIN.
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PMID:Induction, characterization, and cell transfer of autoimmune tubulointerstitial nephritis. 296 68


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