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Query: UMLS:C0155339 (Brown)
 12,436 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Type II alveolar epithelial cells in suspension have been previously shown to possess a Na(+)-H+ antiporter that modulates recovery from an intracellular acid load in the nominal absence of HCO-3 [E. Nord, S. Brown, and E. Crandall. Am. J. Physiol. 252 (Cell Physiol. 21): C490-C498, 1987]. Such a Na(+)-dependent mechanism has also been demonstrated in cultured type II cell monolayers (K. Sano et al. Biochim. Biophys. Acta 939: 449-458, 1988). It has recently been suggested that cultured type II cells possess a H(+)-ATPase that contributes to recovery from an intracellular acid load [R. Lubman, S. Danto, and E. Crandall. Am. J. Physiol. 257 (Lung Cell. Mol. Physiol. 1): L438-L445, 1989]. The present study was undertaken to investigate and characterize the mechanisms by which cultured type II cells recover from an intracellular acid load in the nominal absence of HCO-3. Cultured type II cell monolayers were loaded with the pH-sensitive probe 2',7'-bis(carboxyethyl)-5,6-carboxyfluorescein, and the characteristics of recovery from an imposed intracellular acid load were studied. Recovery of intracellular pH (pHi) was found to be strictly Na(+)-dependent and inhibited greater than or equal to 95% by 1 mM amiloride. Initial rate of recovery was highly sensitive to pHi, with recovery rates varying inversely with increasing pHi. An acidic extracellular pH (6.5) abolished pHi recovery. Treatment of type II cells with either the sulfhydryl reagent N-ethylmaleimide, a nonspecific sulfhydryl reagent, or 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole, a specific vacuolar H(+)-ATPase inhibitor at the concentration tested, resulted in marginal but not statistically significant decrements in pHi recovery. Intracellular ATP depletion, using KCN or replacement of glucose by a nonmetabolizable glucose analogue, reduced pHi recovery by 70-75% relative to control values. Sensitivity to ATP was apparent even under conditions that preserved the transmembrane Na+ gradient. Taken together, these data are most consistent with a single mechanism for pHi recovery in the absence of HCO3-. We interpret this mechanism to be an ATP-sensitive Na(+)-H+ antiporter that acts to reestablish pHi in type II alveolar epithelial cells.
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PMID:ATP-sensitive Na(+)-H+ antiport in type II alveolar epithelial cells. 166 8

1. The concentrations of the oxidized and reduced substrates of the ;malic' enzyme (EC 1.1.1.40) and isocitrate dehydrogenase (EC 1.1.1.42) were measured in freeze-clamped rat livers. By assuming that the reactants of these dehydrogenase systems are at equilibrium in the cytoplasm the [free NADP(+)]/[free NADPH] ratio was calculated. The justification of the assumption is discussed. 2. The values of this ratio obtained under different nutritional conditions (well-fed, 48hr.-starved, fed with a low-carbohydrate diet, fed with a high-sucrose diet) were all of the same order of magnitude although characteristic changes occurred on varying the diet. The value of the ratio fell on starvation and on feeding with the low-carbohydrate diet and rose slightly on feeding with the high-sucrose diet. 3. The mean values of the ratio were calculated to be between 0.001 and 0.015, which is about 100000 times lower than the values of the cytoplasmic [free NAD(+)]/[free NADH] ratio. 4. The differences in the redox state of the two nicotinamide-adenine dinucleotide couples can be explained on a simple physicochemical basis. The differences are the result of equilibria that are determined by the equilibrium constants of a number of highly active readily reversible dehydrogenases and transaminases and the concentrations of the substrates and products of these enzymes. 5. The decisive feature is the fact that the NAD and NADP couples share substrates. This sharing provides a link between the redox states of the two couples. 6. The application of the method of calculation to data published by Kraupp, Adler-Kastner, Niessner & Plank (1967), Goldberg, Passonneau & Lowry (1966) and Kauffman, Brown, Passonneau & Lowry (1968) shows that the redox states of the NAD and NADP couples in cardiac-muscle cytoplasm and in mouse-brain cytoplasm are of the same order as those in rat liver. 7. The determination of the equilibrium constant at 38 degrees , pH7.0 and I 0.25 (required for the calculation of the [free NADP(+)]/[free NADPH] ratio), gave a value of 3.44x10(-2)m for the ;malic' enzyme (with CO(2) rather than HCO(3) (-) as the reactant) and a value of 1.98x10(-2)m(-1) for glutathione reductase.
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PMID:The redox state of free nicotinamide-adenine dinucleotide phosphate in the cytoplasm of rat liver. 439 Oct 39

Hy-Line W-36, W-98, and Brown hens lay approximately the same number of eggs/hen housed to 80 wk; however, little is known about differences in performance during heat stress (HS). Two experiments were performed. The first experiment evaluated intestinal calcium uptake (CaT), heat shock protein-70 (HSP70) liver expression, and endocrine status in the 3 strains under heat stress in response to 1 h of transient exposure to high temperature before onset of 18 h of HS. The second experiment evaluated the differences between W-36 and W-98 in acid-base status observed at 2 different ambient temperatures. The HSP70 and CaT data were analyzed as a completely randomized design (CRD) using a 3 x 2 factorial with strain as a 1 factor and preexposed and control treatments as the other. Estrogen and progesterone data were analyzed as a CRD using repeated measures in a 3 x 2 x 2 factorial with strain as a the first factor, preexposure and control treatments as the second factor, and phase of blood collection as the third factor. The data of the second experiment were analyzed as a CRD using repeated measures in a 2 x 2 x 2 factorial with strain, temperature, and phase of blood collection as the factors. The method applied in both experiments was based on the mixed model (SAS). The results show a strain effect, with the higher CaT in the W-36. The results indicated that transient exposure to HS did not induce changes in HSP70 liver expression. In the second experiment, the blood gas values did not differ between strains, except for the partial pressure of CO(2), in which the values at 22 degrees C are higher for the W-36. At 38 degrees C, there was an increase in blood pH and a reduction in HCO(3)(-) in both strains. The results indicate that endocrine, acid-base status, and Ca homeostasis represent important factors to be considered in assessing genetic differences for thermotolerance.
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PMID:Physiological changes to transient exposure to heat stress observed in laying hens. 1729 67

Tautomerism has been studied conventionally in solutions or in the solid state. However, the importance of mass spectrometry in the gas phase was realized relatively late. 2-Phenacylquinolines are known to undergo ketimine-enaminone tautomerism. The ratio of tautomers is dependent on the nature of the phenyl ring substituent and the Hammett substituent constants sigma. Theoretical calculations indicate the presence of ketimine and enaminone tautomers in the gas phase. The electron ionization mass spectra of eight 2-phenacylquinolines (ketimine form) were recorded at 70 eV in order to determine the fragmentation routes and to screen for the presence of their enaminone tautomers, (Z)-2-benzoylmethylene-1,2-dihydroquinolines, in the gas phase. The relative abundances or total ion currents of some ions correlated with the Hammett substituent constants and Hammett-Brown constants. The product ions [M-CO](+.) and [M-HCO](+) were observed. A reaction mechanism is suggested for the formation of these ions, requiring skeletal rearrangements. The results furnish information relating to tautomerism in the gas phase.
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PMID:Electron ionization mass spectra and tautomerism of substituted 2-phenacylquinolines. 1925 11

Brown algae play a dominant role in the primary productivity of coastal ecosystems and may have an efficient carbon fixation. In this work, 56 genes involved in inorganic carbon fixation were identified from the Saccharina japonica genome. Sequence structure analysis of these genes showed the existence of corresponding function domains and active amino acid sites highly conserved with other stramenopile species. The predicted subcellular localizations showed that Calvin cycle-related enzymes predominantly reside in the plastid and that putative C4-related enzymes are mainly distributed in the mitochondrion. We determined the transcriptional profiles and enzymatic activities of these C4-related enzymes in response to the KHCO3 concentrations and light intensities. Pyruvate orthophosphate dikinase (PPDK) presented the greatest response to low HCO3- concentrations and high light intensity. Phosphoenolpyruvate carboxykinase (PEPCK) was up-regulated at low HCO3- concentrations to compensate for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and might be the crucial decarboxylase in this kelp. We propose that S. japonica might possess a PPDK- and PEPCK-dependent C4-like pathway that enables its rapid growth in natural coastal environments.
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PMID:Genome-wide identification of genes involved in carbon fixation in Saccharina japonica and responses of putative C4-related genes to bicarbonate concentration and light intensity. 3074 83