Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0155339 (Brown)
 12,436 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The binding of thirteen aminoacyl-tRNA synthetases to thirty two immobilised procion dyes has been investigated. Most dyes bind one or more enzymes. The amino acid substrates are not normally potent eluants, with the notable exception of tryptophan eluting tryptophanyl-tRNA synthetase from Brown MX-5BR. Phosphate is frequently extremely effective, much more than expected by simple considerations of ionic strength, indicating that many of the dyes are able to mimic the phosphate groups of the phosphodiester backbone of the nucleic acid. Procedures for the purification of methionyl-, tryptophanyl- and tyrosyl-tRNA synthetases are presented and compared to the conventional purifications of these enzymes. The results indicate the general applicability of these dye columns to the purification of most enzymes of of nucleic acid metabolism and the necessity of investigating as many different dyes as possible for any individual enzyme.
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PMID:The binding of aminoacyl-tRNA synthetases to triazine dye conjugates. 50 62

Brown MX-5BR specifically and irreversibly inactivates tryptophanyl-tRNA synthetase from Bacillus stearothermophilus at pH 8.5. The enzyme is protected from inactivation by the substrates tryptophan and ATP and to lesser extents by ADP, AMP, the product inorganic pyrophosphate and other nucleotides such as GTP. The Kd of the pure reactive dye for the enzyme was measured to be 6.7 X 10(-6) M. The Km values of the two substrates tryptophan and MgATP were found to be 1 x 10(-5) M and 5 x 10(-5) M respectively. The aminated dye is a competitive inhibitor of tryptophanyl-tRNA synthetase with respect to both tryptophan and MgATP with Ki values of 2 x 10(-4) M against both substrates. The use of this dye as an active-site-directed affinity label is discussed.
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PMID:The interaction of tryptophanyl-tRNA synthetase with the triazine dye Brown MX-5BR. 711 37