Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0155339 (Brown)
 12,436 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Proteomic techniques were used to identify cardiac proteins from whole heart homogenate and heart mitochondria of Fisher 344/Brown Norway F1 rats, which suffer protein nitration as a consequence of biological aging. Soluble proteins from young (5 mo old) and old (26 mo old) animals were separated by one- and two-dimensional gel electrophoresis. One- and two-dimensional Western blots with an anti-nitrotyrosine antibody show an age-related increase in the immunoresponse of a few specific proteins, which were identified by nanoelectrospray ionization-tandem mass spectrometry (NSI-MS/MS). Complementary proteins were immunoprecipitated with an immobilized anti-nitrotyrosine antibody followed by NSI-MS/MS analysis. A total of 48 proteins were putatively identified. Among the identified proteins were alpha-enolase, alpha-aldolase, desmin, aconitate hydratase, methylmalonate semialdehyde dehydrogenase, 3-ketoacyl-CoA thiolase, acetyl-CoA acetyltransferase, GAPDH, malate dehydrogenase, creatine kinase, electron-transfer flavoprotein, manganese-superoxide dismutase, F1-ATPase, and the voltage-dependent anion channel. Some contaminating blood proteins including transferrin and fibrinogen beta-chain precursor showed increased levels of nitration as well. MS/MS analysis located nitration at Y105 of the electron-transfer flavoprotein. Among the identified proteins, there are important enzymes responsible for energy production and metabolism as well as proteins involved in the structural integrity of the cells. Our results are consistent with age-dependent increased oxidative stress and with free radical-dependent damage of proteins. Possibly the oxidative modifications of the identified proteins contribute to the age-dependent degeneration and functional decline of heart proteins.
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PMID:Proteomic identification of 3-nitrotyrosine-containing rat cardiac proteins: effects of biological aging. 1534 82

Ethanol reassimilation in Pichia stipitis CBS 6054 was studied by using continuous cultures, and the oxidation of [1-C]ethanol was monitored by in vivo and in vitro C nuclear magnetic resonance spectroscopy. Acetate was formed when ethanol was reassimilated. The ATP/ADP ratio and the carbon dioxide production decreased, whereas the malate dehydrogenase activity increased, in ethanol-reassimilating cells. The results are discussed in terms of the low ethanol tolerance in P. stipitis compared with that in Saccharomyces cerevisiae (S. W. Brown, S. G. Oliver, D. E. F. Harrison, and R. C. Righelato, Eur. J. Appl. Microbiol. Biotechnol. 11:151-155, 1981).
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PMID:Ethanol Reassimilation and Ethanol Tolerance in Pichia stipitis CBS 6054 as Studied by C Nuclear Magnetic Resonance Spectroscopy. 1634 54

In August 2000, several small galls were observed on roots of lady's mantle (Alchemilla acutiloba Opiz) from an old grassland near Kristiansund in northern Norway. These plants, without clear above-ground symptoms, were infected with an unknown root-knot nematode. Soil and plant samples were collected in July 2001 at the previous year's location and sent for identification to the Plant Protection Service in Wageningen, the Netherlands. The smooth elongated galls included several males, swollen adult females with protruding small egg masses and hatching second-stage juveniles. Males and second-stage juveniles also were isolated from adhering soil. The root-knot nematode was identified as Meloidogyne ardenensis Santos (1). Identification was based on female, male, and second-stage juvenile morphology and female isozyme electrophoresis with malate dehydrogenase and esterase. M. ardenensis parasitizes several dicotyledonous hosts, primarily herbaceous and woody plants, and is distributed throughout Europe, with Scotland the most northern area (2). To our knowledge, this is not only the first published report of M. ardenensis in Scandinavia, but also the first report of this species on lady's mantle. References: (1) M. S. N. de A. Santos. Nematologica 13:593, 1968. (2) P. R. Thomas and D. J. F. Brown. Plant Pathol. 30:147, 1981.
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PMID:First Report of the Root-Knot Nematode Meloidogyne ardenensis on Lady's Mantle in Norway. 3083 9