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Query: UMLS:C0155339 (Brown)
 12,436 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Mycoplasma strains of all origins and species can grow on PPLO agar (pleuropneumonia-like organisms), if thallium and L-cystine are added. Brown-black is the coloration of the growing colonies, with the intensity of blackening varying by colony age. Blackening of the mycoplasma colonies is likely to facilitate germ counting, cloning, and evaluation of the growth inhibition test.
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PMID:[Behavior of Mycoplasma strains in media with L-cystine]. 55 51

Acute lung rejection after orthotopic left lung transplantation and Mycoplasma pulmonis infection were studied immunohistologically by bronchoalveolar lavage (BAL) in inbred rats using monoclonal antibodies differentiating lymphocyte and macrophage subpopulations. Twenty transplants in a major histocompatibility complex (MHC)-different strain combination (Brown-Norway/Lewis) were examined 2, 4, and 6 days after transplantation. Thirty isotransplants (Lewis/Lewis) and normal Lewis rats were used as controls. Eight Lewis rats with acute Mycoplasma pulmonis infection and six Lewis rats with chronic Mycoplasma infection also underwent BAL. Mononuclear cell subpopulations were analyzed using a panel of monoclonal antibodies to MHC and macrophage differentiation antigens: ED1 monocyte/macrophages, ED2 inflammatory tissue macrophages, OX19 T lymphocytes, and OX12 B lymphocytes. The following results were obtained: (1) All allotransplants developed acute rejection on day 2, and it advanced until day 6, demonstrating severe perivascular and peribronchiolar infiltration of inflammatory tissue macrophages (ED1+/ED2+): (2) the proportion and number of inflammatory macrophages (ED2+) in BAL fluid increased on day 6; (3) in BAL the proportion and number of T lymphocytes (OX19+) increased more prominently than B lymphocytes (OX12+) on day 6 of acute rejection; (4) in infection with Mycoplasma pulmonis the increase of T lymphocytes (OX19+) in BAL was more prominent than that of B lymphocytes (OX12+). In conclusion, serial analysis of macrophage, T- and B-lymphocyte antigens was performed. The increase of the proportion of inflammatory macrophages (ED2+) and lymphocytes (OX19+, OX12+) in BAL fluid occurred rather late in the rejection response. This limits the use of BAL as an early diagnostic method of allografted lung rejection.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Comparison of mononuclear cell subpopulations in bronchoalveolar lavage fluid in acute rejection after lung transplantation and Mycoplasma infection in rats. 223 Oct 90

Naturally occurring subclinical Corynebacterium kutscheri infection was analyzed by antibody response related to the strain of rats. Wistar-Lewis, Wistar and Spraque-Dawley rats were high responders in seroconversion rates and antibody titers, while Brown Norway and Fischer rats were low responders. The antibody response was related to age also. Some young rats had maternal antibody to C. kutscheri, but antibody disappeared before 8 weeks of age. Rats were antibody-negative for several months thereafter and became antibody-positive after 6 months of age. The antibody response was highest at 8 to 9 months of age in subclinical C. kutscheri infection. This antibody response was very late, compared to the antibody response to Sendai virus and Mycoplasma infections.
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PMID:Naturally occurring subclinical Corynebacterium kutscheri infection in laboratory rats: strain and age related antibody response. 296 78

A study of the bacterial flora of the genitalia of nulliparous Savanna Brown does was carried out both before breeding and at different intervals postpartum to investigate the type of microbial organisms that could be present in the uterus, cervix and the vagina respectively. Of 29 pre-breeding vaginal swabs, Staphylococcus sp. was isolated from 20 goats, Streptococcus sp. from 15 goats and Micrococcus sp. from four goats. Mycoplasma agalactiae was isolated from five goats. The postpartum vagina did not show any appreciable change in the microbial flora, except that Escherichia coli was encountered in two cases. The uterus yielded E. coli from the goats slaughtered 2 days postpartum; Micrococcus sp. from goats slaughtered 12 days postpartum; Staph. aureus from goats slaughtered 16 days postpartum and Staph. aureus from goats slaughtered 24 days postpartum. Also in these two cases-a goat slaughtered at two days postpartum (dpp) and a goat slaughtered 16 dpp-E. coli was present in the uterus. Other isolates from the uteri of slaughtered goats were Micrococcus sp. (12 dpp), Staph. aureus and Micrococcus sp. (16 dpp) and Staph. aureus (24 dpp).
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PMID:Microbiology of the genitalia of nulliparous and postpartum Savanna brown goats. 359 Jun 21

Bailey, Jack S. (George Washington University, Washington, D. C.), Harold W. Clark, William R. Felts, Richard C. Fowler, and Thomas McP. Brown. Antigenic properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area. J. Bacteriol. 82:542-547. 1961.-Antigens were prepared from several tissue culture and human genital strains of pleuropneumonia-like organisms (PPLO) by a method utilizing continuous agitation of the incubating cultures. Antisera were produced in rabbits by intravenous injection of suspensions of these organisms standardized turbidimetrically. The antigenic properties of the selected strains were compared by agglutination techniques supplemented by a test based upon the inhibition of growth of PPLO by specific antisera.The majority of tissue culture strains of PPLO studied, including contaminants from several HeLa cell lines, appeared to be antigenically similar to the human type 1 strains. However, one strain (Sp-1) from a HeLa cell line was found to be related to the human type 2 PPLO.
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PMID:Antigenic properties of pleuropneumonia-like organisms from tissue cell cultures and the human genital area. 1386 15

Bailey, Jack S. (The George Washington University, Washington, D.C.), Harold W. Clark, William R. Felts, and Thomas McP. Brown. Growth inhibitory properties of Mycoplasma antibody. J. Bacteriol. 86:147-150. 1963.-A substance in antiserum responsible for growth inhibition of Mycoplasma was found to be associated with the water-insoluble globulin fraction. This fraction and the agglutinins removed from agglutinated antigen inhibited the growth of Mycoplasma in a similar manner. Sonic oscillation of antiserum broth cultures partially relieved the growth inhibition. The results of this study suggest the existence of neutralized Mycoplasma in a latent phase, which could explain their infrequent detection in tissues and fluids. The application of such techniques as sonic oscillation may improve the recovery and identification of Mycoplasma from these sources.
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PMID:GROWTH INHIBITORY PROPERTIES OF MYCOPLASMA ANTIBODY. 1405 6

Fowler, Richard C. (George Washington University School of Medicine, Washington, D.C.), Don W. Coble, Norman C. Kramer, and Thomas McP. Brown. Starch gel electrophoresis of a fraction of certain of the pleuropneumonia-like group of microorganisms. J. Bacteriol. 86:1145-1151. 1963.-Starch gel electrophoresis is suggested as a means of further characterizing strains of the pleuropneumonia-like group of microorganisms (PPLO), defined herein as including both the various "L" forms of bacteria and the Mycoplasmataceae. The techniques used may be varied to "select" other groups of proteins or classes of compounds (e.g., glycoproteins, lipoproteins, and enzymes). Only the results by starch gel electrophoresis on the soluble portion of sonically treated, washed cultures, grown in a pancreatic digest of beef heart enriched with bovine serum, are reported. As yet, differences appear more significant than similarities among the electrophoretically separable proteins. The demonstration of characteristic protein patterns for each strain studied suggests possibilities of further dividing serological groups into sero-logically distinct subtypes. Tentative migration values (E(f)) have been assigned to the protein bands for convenience in designation. Numerous interrelationships are thereby suggested.
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PMID:STARCH GEL ELECTROPHORESIS OF A FRACTION OF CERTAIN OF THE PLEUROPNEUMONIA-LIKE GROUP OF MICROORGANISMS. 1408 81

The pathogenesis of Mycoplasma pneumoniae infection was studied in the Syrian hamster with qualitative and quantitative culture methods and special histopathologic techniques. The animals were readily infected with the mycoplasma, which multiplied throughout the respiratory tract. Sensitivity of this experimental host to infection was indicated by the 50 per cent infective dose, which was 10 colony-forming units of the organism. Inoculation consistently resulted in the production of peribronchial pneumonitis which was induced by the mycoplasma. The organisms were visualized in a superficial location in the mucosa of involved bronchi, by means of indirect fluorescent antibody staining and by a modification of the Brown and Brenn technique. The data indicate applicability of the hamster to the study of problems concerned with M. pneumoniae disease which are impractical or impossible to resolve in the human host.
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PMID:EXPERIMENTAL INFECTION WITH MYCOPLASMA PNEUMONIAE (EATON'S AGENT). 1431 3

Clark, Harold W. (The George Washington University, Washington, D.C.), Jack S. Bailey, Richard C. Fowler, and Thomas McP. Brown. Identification of Mycoplasmataceae by the fluorescent antibody method. J. Bacteriol. 85:111-118. 1963.-The conditions of the fluorescent antibody reactions were studied in relation to their application to Mycoplasmataceae or pleuropneumonia-like organisms (PPLO). Mycoplasma hominis type 1 and 2 antigens and their homologous antisera were used to determine the activity and specificity of these and other strains. Fluorescein isothiocyanate conjugated antiserum globulin preparations were used in both the direct and indirect fluorescent antibody methods. A direct tube technique was used for the detection and measurement of growth in broth cultures by the addition of conjugated antiserum. The specific fluorescent staining and recognition of hot water fixed M. hominis colonies was presented as a suitable identification standard. The antigenic activity was found to remain in the insoluble residue after exposure of M. hominis strains to sonic vibration (9 kc) for 30 min and centrifugation. Brief 2-min exposures of tissue cells to vibration (9 kc) caused the disruption of tissues, with the release of viable and "bound" nonwashable strains that reacted specifically with fluorescent antibody. It is proposed to apply both the sonic vibration and the fluorescent antibody techniques for the identification of Mycoplasmataceae in human tissues.
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PMID:IDENTIFICATION OF MYCOPLASMATACEAE BY THE FLUORESCENT ANTIBODY METHOD. 1656 85

Approximately 20% of outbreaks of amyloid arthropathy in The Netherlands were earlier attributed to Enterococcus faecalis but there was no explanation for the remaining cases. In a further study, material from the joints of 10 birds from each of 10 affected brown layer flocks and two broiler flocks was cultured in several bacteriological media. E. faecalis was isolated from one layer flock but Mycoplasma synoviae was recovered from purulent joints in six of the layer flocks and both broiler flocks. Brown layers were then infected experimentally with one of the M. synoviae isolates to assess its arthropathic and amyloidogenic potential. Using Congo red staining, articular amyloid deposits were seen 12 weeks after intra-articular or intravenous inoculation in almost all birds. After intra-articular inoculation of the left knee joint the contralateral hock tendon sheath tended to be affected, while hock and foot joints were mainly affected after intravenous inoculation. Amyloid deposits were seen in livers and spleens of many birds. This is the first demonstration of arthropathic strains of M. synoviae with an association with amyloid arthropathy, while for one isolate its amyloidogenic potential was shown experimentally.
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PMID:Field studies on the association between amyloid arthropathy and Mycoplasma synoviae infection, and experimental reproduction of the condition in brown layers. 1918 57


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