Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0153690 (
bone metastases
)
6,382
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We analyzed
glyceraldehyde-3-phosphate dehydrogenase
(
GAPDH
) expression in normal and malignant human prostate tissues, normal rat prostate, and Dunning R-3327 rat prostate cancer cell lines. We detected multiple forms of
GAPDH
in Dunning cell lines by two-dimensional protein electrophoresis and Western analysis. Five forms of
GAPDH
that differed by isoelectric point were detected for each of the two metastatic Dunning cell lines, while four or fewer forms were detected for Dunning cell lines with low metastatic ability. We also detected multiple forms of
GAPDH
in normal and malignant human prostate specimens by two dimensional protein electrophoresis and immunohistochemical analysis.
GAPDH
was undetectable in normal human prostate secretory epithelium by immunohistochemistry, but was abundant in nuclei of normal basal cells and stromal cells. In human prostate cancer specimens, there was a rough correlation between cytoplasmic staining for
GAPDH
and tumor grade, but
GAPDH
staining was extremely heterogeneous.
GAPDH
was abundant in nuclei of some high-grade human prostate tumors. Both of the human prostate cancer
bone metastases
analyzed with immunohistochemistry had markedly elevated cytoplasmic
GAPDH
expression. We conclude that multiple forms of
GAPDH
may play diverse roles in normal prostate tissue and in prostate cancer.
...
PMID:There are multiple forms of glyceraldehyde-3-phosphate dehydrogenase in prostate cancer cells and normal prostate tissue. 865 74
