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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spleen lymphocytes and erythrocytes from congenic mice of diffrent haplotypes were characterized on a precise biophysical basis (the anodic electrophoretic mobility, EPM) to correlate any subtle differences in the cell surface topochemistry with the H-2 specificity. Spleen T lymphocytes from A.CA (H-2f) and A.SW (H-2s) mice exhibited high values EPM, which were significantly different. In contrast, significant differences in the EPM of B cells and erythrocytes were not observed. Cell electrophoresis of spleen T lymphocytes (without " contaminating " B cells), before and after the chemical modification of the cell surface by treatment with small/non-toxic concentrations of maleic anhydride, showed the number of lysine side chain amino-groups in the periphery of cells with H-2f specificity to be about twice those on cells with H-2s haplotype. Such a difference was observed both in the case of premature T lymphocytes and mature T lymphocytes. The difference in the number of cationogenic amino-groups in the cell periphery contributing a positive charge, would explain the oberved difference in the EPM of H-2f and H-2s spleen T lymphocytes, and suggest that the macromolecules coded by the H-2 genes or other genes under H-2 control lead to delicate differences in the chemical composition of the surface membranes of cells of the two H-2 haplotypes, expressed only on high EPM lymphocytes of spleen (T or T-like cells).
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PMID:[Genetic control of the H-2 region of the cell surface: cationic amino groups in the periphery of T lymphocytes]. 30 May 97

Spleen cells from C3H/Hej mice (H-2k) respond poorly to the B-cell mitogen lipopolysaccharide in vitro as compared to the related strains C3H/Tif (H-2k) and CBA/Orl (H-2k). The electrokinetic properties of splenic lymphocytes from these 3 strains were investigated in parallel, in order to both quantitate low-mobility B cell and high-mobility T cell populations and measure their mean electrophoretic mobilities. C3H/Hej mice were found to possess the same proportion (55%) of LM cells as C3H/Tif and CBA/Orl mice. Therefore, the low LPS-responsiveness of C3H/Hej is not due to a numerical deficiency in B cells. Whereas the mean EPM of HM cells was identical in the 3 strains, that of LM cells was slightly (6%) but significantly (Student's t test, P less than 0.01) lower in C3H/Hej than in the high LPS-responder controls. This suggests that the membrane-structure required for activating interaction with LPS might contribute to B-cell electronegative surface-charge.
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PMID:Electrokinetic properties of splenic lymphocytes from the low-lipopolysaccharide responder C3H/Hej mice. 108 4