Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

An alternative model for corneal allografting termed the reverse corneal allograft reaction (RCAR) was developed in this study. Spleen cells from an alloimmunized donor were injected into the corneal stroma of the immunizing donor strain or were restimulated in mixed lymphocyte culture and then injected into the corneal stroma of the immunizing strain. The reaction began as a circular opaque site that spread and became irregularly shaped during the first 5 days after cell injection. The epithelial surface of the cornea became uneven and epithelial cell erosions were noted. Histological examination revealed that corneal stromal keratocytes at the site of inoculation had undergone degeneration and the injected cells had migrated toward the epithelial-stromal boundary, wherein a disruption of the basement membrane and disintegration of the epithelial cells occurred. Purified spleen cell subsets injected separately did not mediate the reaction. A suspension of T lymphocytes and class II antigen-positive macrophagelike cells was required to cause the RCAR. This reaction, which mimics a delayed-type hypersensitivity response, was transient, reaching a peak by day 5 and waning by day 8. This experimental model of the corneal allograft reaction shows promise for the study of cells and mediators of the corneal allograft reaction and can be employed as a reproducible system in which to test drug therapies for the treatment of corneal allograft rejection.
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PMID:Cell-mediated immunity in the cornea. 297 Jan 33

In order to analyse migration patterns of donor MHC class II cells out of transplanted kidney and accumulation of host cells within the graft, immunomorphological studies were performed using monoclonal antibodies in rat allogeneic kidney transplantation model. To answer the question of how many donor cells migrate out of the renal cortex MRC 0 x 3 monoclonal antibody (MoAb) against LEW MHC class II antigens was used. In the grafts explanted after 4,24 48 and 73 h, a slow reduction of donor class II cells was observed and some areas in cortex showed only very few, if any, donor cells. At the same time, starting from day 2 after transplantation accumulation of donor cells was found in perivascular spaces. Spleen sections stained at 24, 48, 72 and 96 h after transplantation revealed donor cells present in recipient's spleen. They were detected up to day 3 after surgery. Their numbers, however, decreased after day 2. After 2 and 3 days, accumulations of recipient's cells between tubules were detected. It was found that many cells in infiltrations were stained with anti-T lymphocyte MoAb. Expression of class II antigen on rat kidney cells increases significantly from the day 4 after transplantation.
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PMID:Cell migration between graft and host--an analysis with monoclonal antibodies after allogeneic rat kidney transplantation. 874 1