Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Monoclonal antibodies (MAbs) were sought that would identify Streptococcus suis. Spleen cells from Balb/c mice immunized with formalin-killed S. suis were fused with the murine cell-line SP-2/0. Hybridomas positive for S. suis were identified by a solid-phase radioimmunoassay with a mixture of all available serotypes of S. suis. On further screening with individual serotypes of S. suis (Types 1, 2, 1/2, 3, 4, 5, 7, 8, 9 and 12) and other related and unrelated bacteria, a hybridoma specific for Type 1 was identified. Another hybridoma was positive for all serotypes of S. suis. S. suis Type 1-specific MAbs in the form of ascites fluid was used in coagglutination test. Agglutination with S. suis Type 1 was observed within 45 s, while there was no reaction with other serotypes of S. suis or other bacteria, during the 5-min observation period. A library of type-specific MAbs should help in rapid and accurate serotyping of S. suis isolates.
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PMID:Monoclonal antibodies for coagglutination of Streptococcus suis type 1. 280 Mar 8

Spleen cells from Balb/cJ mice inoculated with bovine immunoglobulin (Ig)G were hybridized with hypoxanthine aminopterin thymidine-sensitive nonsecreting cell line SP-2/0, and the hybrid cell culture fluid was tested for specificity. Hybrid cells secreting monoclonal antibodies to bovine IgG were isolated and recloned. The monoclonal antibody DAS 2 was specific for bovine IgG2 by an indirect solid-phase radioimmunoassay. This clone was adapted for growth as antibody-secreting neoplasms in Balb/cJ mice, the sera from which contained high titers (1:128,000) of anti-bovine IgG2 antibodies. Antibody specific to bovine IgG2 was isolated by affinity chromatography. This antibody was shown by Ouchterlony analysis to be mouse IgG1 with the kappa light chain. On isoelectric focusing, this antibody gave a pattern that was consistent with monoclonality.
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PMID:Production and characterization of monoclonal antibodies to bovine immunoglobulin G2. 709 14