Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spleen cells (SpC) from nonimmunized CFW mice were converted into antibody plaque-forming cells (PFC) by incubation with RNA extracted from livers and spleens of immunized mice (4 days after a single intravenous injection of 0.2 ml of 5% sheep red blood cells (SRBC). RNA was extracted by the phenol-detergent procedure only when sensitization determined by the technique of Jerne showed at least one PFC per 1,500 SpC. Immunogenic activity of RNA from lives of immunized mice was identical with that of RNA from spleens. Immunogenic RNA was inactivated by RNase but not by DNase or pronase, indicating that induction of antibody synthesis requires intact RNA. Newly synthesized antibodies were specific for the SRBC injected antigen; plaques did not occur when other RBC were used in place of SRBC for the in vitro test. The influence of antibiotics on this phenomenon is also discussed.
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PMID:Induction of antibody synthesis in vitro by immunogenic RNA. 119 43

Spleen cells from leukemic AKR mice contain nonspecific T suppressor cells that suppress the in vitro anti-SRBC responses of normal AKR spleen cells. The suppression is abrogated by pretreatment of the leukemic cells with DNase. Cells with surface DNA can be enriched by treatment with anti-DNA antibodies and removed on anti-Ig columns. Even though there is a 10-fold enrichment in suppression by the surface DNA-positive cells (DNA+), the depleted population (DNA-) still suppresses with an efficiency comparable to the unfractionated spleen cells. Pretreatment with anti-I-J antiserum and separation on columns neither enriches nor depletes suppressor cells. Fractionating leukemic spleen into 4 populations of cells by sequential treatment with anti-DNA and anti-I-J with separation on appropriate columns, resulting in 4 populations (DNA+/I-J-, DNA+/I-J+, DNA-/I-J+, and DNA-/K-J-), revealed that the DNA+/I-J- cells that comprise ca. 15% of the total are the most potent suppressors. The DNA+/I-J+ cells that comprise ca. 2% of the cells are less efficient and their suppression is not sensitive to DNase. The DNA-/I-J+ population (ca. 10% of the total) are also poor suppressors. The remaining cells, which are DNA-/I-J-, do not act as suppressors. When small numbers of any 2 of the 4 fractions were added together, it was found that the DNA+/I-J- cell can interact with the DNA+/I-J+ and the DNA-/I-J+ cells but not the DNA-/I-J- cells to give enhanced suppression.
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PMID:Cell surface DNA in immunosuppression in AKR leukemia. 700 Aug 99

Diets containing unheated casein (CD; control) or a casein-glucose mixture (CGD) previously heated at 140 degrees for 2 h were fed to two groups of young rats for 21 d. Differences in body weight, feed consumption, thymus, and spleen growth, protein metabolism and in vivo immune response were then determined. For this last experiment, animals were inoculated with sheep erythrocytes (SRBC) on day 15 to provide an immunological challenge. No changes were seen in body weight, feed consumption or feed conversion ratios. Neither were significant differences found in spleen weight, protein content, DNA content, DNase (EC 3.1.4.6) activity or lymphocyte count, suggesting that spleen cell growth remained similar in all the animals studied. The CGD induced marked increases in thymus DNA content whilst the protein:DNA ratio became lower. Spleen RNA content was similar in all rats, but thymus RNA content was 29% lower in the CGD group, although this difference did not reach statistical significance. This fact might be a consequence of the low RNase (EC 2.7.7.16) activity and RNase:RNA ratios in the thymus glands of CGD-fed animals. Further, the number of splenic plasma cells secreting anti-SRBC antibodies (direct plaque-forming cells) was significantly decreased in the same group. It might be concluded that both diets are adequate for rat growth and that the differences observed in the thymus of CGD-fed rats may be directed towards preserving tissue function. Nevertheless, the CGD did cause immunological disturbances affecting the humoral immune response.
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PMID:Immunocompetence in relation to a heat-processed diet (Maillard reaction) in weanling rats. 922 91