Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

CRF is a primary integrator of the organism's coordinated neuroendocrine, autonomic, behavioral, and immune responses to stress. In the present study the identity of the cell type(s) expressing CRF receptors in mouse spleen was determined using a combination of cell fractionation and receptor-binding techniques. Autoradiographic studies of the distribution of [125I]Tyro-ovine CRF [( 125I]oCRF)-binding sites in spleen localized CRF receptors primarily to the red pulp and marginal zones. The distribution pattern of [125I]oCRF-binding sites closely resembled the pattern of India ink accumulated in phagocytic cells in the same sections. To identify the specific cell type(s) expressing CRF receptors, [125I]oCRF-binding activity was evaluated in splenic cell populations fractionated on the basis of their physical and functional properties. Macrophages were identified in each fraction by their phagocytosis of polystyrene beads and membrane labeling with MONTS-4, a monoclonal antibody specific for resident macrophages. Spleen cells were fractionated by adherence to glass bead or Sephadex G-10 columns, phagocytosis of carbonyl iron particles, and centrifugation on discontinuous Percoll gradients. By all fractionation methods, there was a significant correlation of [125I]oCRF binding with both phagocytic activity (r = 0.75; P less than 0.001) and MONTS-4 staining (r = 0.84; P less than 0.001), strongly suggesting that CRF receptors are primarily expressed on resident splenic macrophages. However, there was essentially no specific binding of [125I]oCRF to either resident or elicited peritoneal macrophages or to several monocyte/macrophage, B-cell, or T-cell lines. While these results suggest that the expression of CRF receptors may be restricted to a population of splenic macrophages, they do not exclude the possibility that CRF receptors may be induced on resident macrophages in spleen and other immune system-related tissues by factors present in the microenvironment.
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PMID:Corticotropin-releasing factor receptors in mouse spleen: identification of receptor-bearing cells as resident macrophages. 216 23

Murine monoclonal antibodies against human/rat corticotrophin-releasing factor-41 (CRF-41) were produced and characterized for use in the immunological and biological characterization of CRF-41. Spleen cells from BALB/c mice immunized with CRF-41 conjugated to bovine gamma-globulin were fused with a BALB/c-derived non-secretor X-63 myeloma line. Hybridomas were selected for CRF antibody production by enzyme-linked immunosorbent assay, and positive hybridomas cloned twice. Three monoclonal antibodies were obtained (KCHMB001, KCHMB002 and KCHMB003) and characterized as IgG1, IgG1 and IgG2a isotypes respectively, with affinity constants for rat CRF-41 of 30, 53 and 34 nmol/l respectively. All three monoclonal antibodies recognize an epitope contained between residues 34 and 41 of the human/rat sequence. The antibodies were able to neutralize the ACTH-releasing activity of rat CRF-41, applied to rat pituitary fragments in vitro, in a dose-dependent manner. Isoelectric focusing showed that KCHMB003 detected bands of synthetic rat CRF-41 and rat [Met(O)21,38]-CRF-41 at pH 7.1 and 6.8 respectively. Use of KCHMB003 in a two-site enzyme-amplified immunoassay showed that this antibody recognizes both synthetic rat CRF-41 and immunoreactive CRF-41 in rat hypothalamic tissue extracts.
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PMID:Production and utilization of monoclonal antibodies to human/rat corticotrophin-releasing factor-41. 224 88