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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spleen cells from mice immunized with a variety of antigens and incubated in vitro with killed spherules of Coccidioides immitis lyse six to eight times more autologous murine erythrocytes than normal spleen cells and spherules. Cellular and biochemical events in this phenomenon were investigated to ascertain its significance. Kinetic studies suggested that hemolysis results from the activation of some immune cells by spherules. The capacity of spherules to activate these cells is rather unusual because, of the inert particles tested, only zymosan A and crude chitin demonstrated comparable activity. Furthermore, although the hemolytic phenomenon occurred in serum-free medium, more lysis was effected by immune cells and opsonized spherules or zymosan A than by immune cells and untreated fungal particles. Sheep, chicken, and human erythrocytes were not lysed in the hemolytic phenomenon; however, hemoglobin in chicken and sheep erythrocytes was oxidized. Both the murine erythrocyte lysis and oxidation of ovine hemoglobin correlated with the reduction of Nitro Blue Tetrazolium by immune cells adherent to spherules, and both phenomena appeared to be mediated by H2O2 released into the medium by activated cells. Spleen cells reactive with spherules could not be depleted by treatment with iron carbonyl, antiimmunoglobulin plus complement, or anti-brain-associated theta plus complement, but they were partially or completely depleted after rosette formation with erythrocytes coated with antibody or murine complement. Using light and electron microscopy, we noted that immune spleens contained more neutrophils than normal spleens, that these neutrophils reduced Nitro Blue Tetrazolium after stimulation with phorbol myristate acetate, and that they were the most prevalent cell type adherent to spherules after incubation in vitro.
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PMID:Significance of an in vitro phenomenon in which murine erythrocytes are lysed by autologous spleen cells and spherules of Coccidioides immitis. 37 5

Separation by gel-filtration chromatography and enzyme assay with H2O2 and an organic hydroperoxide substrate were used to identify selenium (Se)-dependent and non-Se-dependent glutathione peroxidase (GSH-Px) in tissues of calves fed supplemented whole milk diets differing only in their Se content (0.03, 0.23, and 0.53 microgram/g of total solids). Spleen, cardiac muscle, erythrocytes, brain, thymus, adipose tissue, and striated muscles of calves were found to contain only the Se-dependent GSH-Px. Tissues having both enzymes included liver, lungs, adrenal glands, testes, kidney medulla, and kidney cortex. Hepatic tissue contained the highest percentage of non-Se-dependent GSH-Px activity of the calf tissues. For those tissues having Se-dependent and non-Se-dependent GSH-Px activity, the Se-dependent enzyme activity was generally lower for calves fed 0.03 microgram of Se/g of total solids as compared with activity in calves fed larger amounts of Se. The tissue ranking of non-Se-dependent GSH-Px activity, as a percentage of total GSH-Px activity, was not changed by differences in the Se content of the diets fed to the calves.
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PMID:Distribution of selenium-dependent and nonselenium-dependent glutathione peroxidase activity in tissues of young cattle. 732 34