Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spleen cells from mice immunized with human cells were transfected with DNA from the human leukemia cell line, Reh. A calcium phosphate-DNA coprecipitate was introduced into the stimulated spleen cells by treatment with a polyethylene glycol-DMSO mixture. The cells which grew out from the transfected population could be passaged continuously in culture and cloned in semisolid agarose. The cell lines contain 40 acrocentric chromosomes, and Southern blot analysis with the cloned human Alu sequence indicates that human DNA is present. The transfected cell lines exhibit markers expressed on plasmacytoma cells and produce immunoglobulin in amounts equivalent to those produced by plasmacytoma cell lines. Five of nine cell lines tested produce antibodies that react with the human cells used to immunize the mice. These cell lines have been in culture for more than a year, and one of the lines has maintained a diploid karyotype and production of the specific antibody even after being passaged through a BALB/c mouse. Preliminary experiments indicate that these cells may be a useful model system for analysis of the early proliferative phase of leukocyte transformation.
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PMID:Production of continuous mouse plasma cell lines by transfection with human leukemia DNA. 659 40

We sought to determine if the histone deacetylase inhibitor (HDI), trichostatin A (TSA), would alter systemic lupus erythematosus (SLE) in NZB/W mice. Fourteen to sixteen-week-old female NZB/W F1 mice were given TSA (1.0mg/kg body weight (BW)) intraperitonealy (i.p.) daily, TSA (1.0mg/kg BW) i.p.+anti-CD25 (250mg/mouse) i.p. every third day, only anti-CD25 (250mg/mouse) i.p., DMSO or isotype IgG. Disease progression was assessed as they aged. Mice were sacrificed at 26 or 38 weeks of age, tissues collected and evaluated. At 36 weeks, TSA-treated animals had decreased anti-double stranded DNA (dsDNA) autoantibodies and decreased protein excretion compared to controls. Spleen size and the percentage of CD4+CD69+ cells were decreased, with an increase in CD4+CD25+ T cells in the TSA-treated mice. Real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis of T cells showed a decrease in IL-6 production but an increase in TGF-beta1 and Foxp3 in the TSA-treated animals. Kidney analysis showed a decrease in IgG and C3 deposition, decrease in pathologic glomerular disease and renal MCP-1, MMP-9, and IL-6 mRNA expression. Anti-CD25-treated mice euthanized at 26 weeks of age showed decreased Foxp3+CD4+CD25+ T cells compared to TSA-treated mice. These data suggest TSA administration modulates lupus-like disease, in part, by increasing T regulatory cells.
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PMID:The histone deacetylase inhibitor trichostatin A upregulates regulatory T cells and modulates autoimmunity in NZB/W F1 mice. 1865 65