Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of in vitro interferon stimulation on nonimmune- and immune-spleen natural killer cell activity was studied in iron-deficient rat pups. Dams were fed 6, 12 or 250 mg Fe/kg diet during gestation and lactation. Approximately one-half of the 17-d-old pups were injected intraperitoneally with 10(5) plaque-forming units of vaccinia virus. Four d later, nonimmune and vaccinia-immune pups were killed. Spleen lymphocyte suspensions were prepared and plated with or without rat alpha/beta interferon for 2 h at 37 degrees C. Washed lymphocytes were combined with 51chromium-labeled YAC-1 target cells and co-cultured for 4 and 16 h at 10 and 50:1 effector-to-target ratios. Hemoglobin concentration, hematocrit, body weight, spleen weight and lymphocyte numbers per spleen were lower in iron-deficient pups than in controls. In general, interferon stimulation increased natural killer cell activity above baseline. Analysis of variance comparison among groups showed that interferon was incapable of restoring natural killer cell activity of iron-deficient pups to the levels observed in control pups. Impaired spleen natural killer cell activity in iron-deficient neonates may be due to a limited capacity for stimulation by interferon.
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PMID:Spleen natural killer cells from iron-deficient rat pups manifest an altered ability to be stimulated by interferon. 246 45

We investigated the effect of 15-deoxyspergualin (DSG) on graft rejection, starting administration at the onset of rejection and on the induction of immunologic unresponsiveness. Hearts from WKAH rats were transplanted into the neck of ACI rats. The energy metabolism of the grafted hearts was followed by 31P nuclear magnetic resonance spectroscopy. The day that energy metabolism started to fall was defined as the onset of rejection, and intraperitoneal administration of DSG was initiated at 5 mg/kg/day for 15 days from this day. The grafted heart arrested in 2 of 10 rats 9 and 11 days after transplantation, respectively, but the remaining 8 recovered from rejection and 5 of them showed evidence of immunologic unresponsiveness. Of 10 rats treated with DSG from the day of transplantation, only 1 rat showed evidence of unresponsiveness. The initiation of DSG treatment from the onset of rejection resulted in a higher percentage of induction of unresponsiveness. Therefore, DSG was considered to specifically inhibit lymphocyte clone expansion at the onset of rejection. Spleen cells obtained from recipients 7-10 days after the end of DSG treatment were administered to syngeneic ACI rats grafted with WKAH hearts. Graft survival was significantly prolonged, but long-term unresponsiveness could not be transferred. However, immunologic unresponsiveness could be adoptively transferred in 3 of 5 rats receiving spleen cells from syngeneic rats that had recovered from rejection after DSG treatment and had acquired long-term unresponsiveness. These results suggest that suppressor cells are resistant to DSG and are spared and participate in the maintenance of immunologic unresponsiveness.
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PMID:Therapeutic effect of 15-deoxyspergualin on acute graft rejection detected by 31P nuclear magnetic resonance spectrography, and its effect on rat heart transplantation. 297 69

Spleen cells from Balb/cJ mice inoculated with bovine immunoglobulin (Ig)G were hybridized with hypoxanthine aminopterin thymidine-sensitive nonsecreting cell line SP-2/0, and the hybrid cell culture fluid was tested for specificity. Hybrid cells secreting monoclonal antibodies to bovine IgG were isolated and recloned. The monoclonal antibody DAS 2 was specific for bovine IgG2 by an indirect solid-phase radioimmunoassay. This clone was adapted for growth as antibody-secreting neoplasms in Balb/cJ mice, the sera from which contained high titers (1:128,000) of anti-bovine IgG2 antibodies. Antibody specific to bovine IgG2 was isolated by affinity chromatography. This antibody was shown by Ouchterlony analysis to be mouse IgG1 with the kappa light chain. On isoelectric focusing, this antibody gave a pattern that was consistent with monoclonality.
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PMID:Production and characterization of monoclonal antibodies to bovine immunoglobulin G2. 709 14