Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spleen cells, sensitized in vitro with ANF-KLH conjugate, were fused with a non-producing mouse myeloma cell line, X63-Ag8.653. One of the isolated hybridomas secreted a monoclonal antibody which exhibited an association constant of 7.25 X 10(-9) M for ANF and recognized an epitope at the C-terminal of the synthetic rat ANF(101-126). Cross reactivity experiments with Auriculin B, Atriopeptin I and Atriopeptin II suggests Phe124-Arg125-Tyr126 in the epitope recognized by this antibody. The epitope appears to be similar to the portion of the hormone molecule essential for ANF binding to the B-receptor (high m.wt. receptor). This antibody may be useful for the generation of anti-idiotypic antibodies for the B-receptors.
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PMID:Production of a monoclonal antibody against C terminus of atrial natriuretic factor by in vitro immunization. 252 33