Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

BALB/c mice immunized with Nonidet P-40 (NP-40) crude solubilized (CS) extracts of a syngenetic methylcholanthrene-induced BALB/c sarcoma (Meth A) were challenged with viable Meth A cells to determine the ability of the solubilized preparations to induce transplantation rejection. Animals resisting such challenge were then used in agarose microdroplet macrophage migration inhibition (MMI) and tumor cell neutralization (Winn) assays to evaluate the antigenic specificity of these CS extracts. Spleen cells from those animals that rejected Meth A after immunization with the NP-40-solubilized preparations effectively neutralized the tumor-producing capacity of Meth A tumor cells as determined in Winn assays. MMI assays were quite sensitive and detected migration inhibition of peritoneal exudate (PE) cells from immunized mice with extract concentrations as low as picogram quantities. Specificity studies demonstrated that Meth A expressed no antigenic cross-reactivity with similarly prepared extracts of an unrelated SV40-induced sarcoma (mKSA), nor with a mineral oil-induced plasmacytoma (ADJ-PC5) of BALB/c mice. Inhibition of PE cell migration was mediated by culture supernatants (presumably migration inhibition factor [MIF]) generated from a mixture of immune spleen cells and mitomycin C (MMC)-treated Meth A cells as assayed in an indirect MMI test.
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PMID:Cellular immunity to solubilized tumor antigens of a methylcholanthrene-induced sarcoma with a migration inhibition assay. 65 88

Migration inhibition activity from ascitic fluids of ovarian cancer patients (OC-MIF) was used to develop monoclonal antibodies. The OC-MIF was purified about 10,000 fold by affinity chromatography on L-fucose-Sepharose 6B. Spleen cells from AB/Jena mice immunized with purified OC-MIF were hybridized with P3X63 Ag 8.653 myeloma cells. Supernatants of the hybridoma cultures were screened by solid-phase binding assay, direct neutralizing assay and solid-phase RIA. Several clones of these hybridomas secreted antibodies into the culture medium, which neutralized the biological activity of OC-MIF at dilutions as high as 10(-4) relative to the initial culture medium. After expansion and cloning one clone was selected for ascitic antibody production. This monoclonal antibody coupled to Sepharose 4B adsorbed OC-MIF. Most of the adsorbed biological activity could be eluted with 0.1 M acetic acid.
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PMID:Inhibition of macrophage migration by a factor from ascites fluids of ovarian cancer patients. III. Generation of monoclonal antibody specific for human MIF. 331 Oct 40

Spleen cells from hyperimmunized mice infected with Toxoplasma gondii were cultured in vitro with Toxoplasma specific antigen. The supernatant produced from the cells were termed lymphokines (LKs). The LKs were divided into 4 major fractions, namely: LKs-I, LKs-II, LKs-III and LKs-IV, according to the elution pattern on Sephadex G-100 gel columns. Partially purified LKs contained 2 MIF peaks, namely: MIF-I in LKs-II fraction and MIF-II in LKs-IV fraction. In this study, Toxoplasma growth inhibitory factor (Toxo-GIF), which inhibits the multiplication of Toxoplasma within non-immune macrophages in vitro, was separated by the same method as MIF separation, i.e. Sephadex G-100 gel filtration. Toxo-GIF activity was present in the LKs-II fraction in which MIF-I was also detected with a calculated molecular weight of 30,000 to 40,000. This murine LKs inhibited Toxoplasma multiplication only in murine macrophages but not in guinea pig macrophages or canine monocytes. Cytotoxic substances against macrophages were observed in the LKs-IV fraction, however, no Toxo-GIF was present in this fraction which in addition contained MIF-II with a calculated molecular weight of 3,000 to 5,000.
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PMID:Mouse spleen cell-derived toxoplasma growth inhibitory factor: its separation from macrophage migration inhibitory factor. 700 83