Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Spleen cells from C3H/Hej mice (H-2k) respond poorly to the B-cell mitogen lipopolysaccharide in vitro as compared to the related strains C3H/Tif (H-2k) and CBA/Orl (H-2k). The electrokinetic properties of splenic lymphocytes from these 3 strains were investigated in parallel, in order to both quantitate low-mobility B cell and high-mobility T cell populations and measure their mean electrophoretic mobilities. C3H/Hej mice were found to possess the same proportion (55%) of LM cells as C3H/Tif and CBA/Orl mice. Therefore, the low LPS-responsiveness of C3H/Hej is not due to a numerical deficiency in B cells. Whereas the mean EPM of HM cells was identical in the 3 strains, that of LM cells was slightly (6%) but significantly (Student's t test, P less than 0.01) lower in C3H/Hej than in the high LPS-responder controls. This suggests that the membrane-structure required for activating interaction with LPS might contribute to B-cell electronegative surface-charge.
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PMID:Electrokinetic properties of splenic lymphocytes from the low-lipopolysaccharide responder C3H/Hej mice. 108 4

We have recently shown [Kiefer H, Blume AJ & Kaback HR (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 2200-2204] that depolarization of the lymphocyte membrane constitutes an early event in mitogenic stimulation. We now present evidence that these early changes in membrane potential occur only as a consequence of specific binding of lipopolysaccharide (LPS) or concanavalin A (Con A) to cells displaying the correct functional receptors. Spleen cells from LPS low-responder mice (C3H/HeJ) show no depolarization with LPS but only with Con A, while those of the LPS high-responder (C3H/Tif Bom) depolarize upon addition of LPS and Con A. Moreover, in T cells, depleted of I-A-bearing cells, the depolarization produced by a specific stimulus is only reversed by the addition of growth factors, thus indicating that mitogenic activation of lymphocytes is at least a two-signal mechanism. These early events could more recently be correlated with changes in intracellular pH [Gerson DF, Kiefer H & Eufe W (1982) Science 216, 1009-1010; Gerson DF & Kiefer H (1982) J. Cell. Physiol., in press].
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PMID:The membrane potential of lymphocytes changes only in response to specific stimulation. 713 73