Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0153470 (Spleen)
 4,015 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Tumor-specific transplantation antigens unique to each of MM2, MM46 and Ehrlich mouse ascites tumor cells (cell line-specific antigens) were released from the cells into the medium during incubation in 0.12M saline at 37 degrees for 30 min. The released antigens were purified by identical procedures which consisted of ultracentrifugation, affinity chromatography using Sepharose 4B conjugated with Ricinus communis lectin, and DEAE-cellulose column chromatography. The recovery was about 700 micrograms (protein) from 100 g (wet weight) cells. The recovered materials induced specific immunity in C3H/He mice against transplantation of the corresponding tumor cells only when they were administered after treatment with the corresponding tumor-regressor C3H/He mouse serum. Single injection into the peritoneal cavity of 10 mcrograms of each of the pretreated materials inhibited transplantation of 5 x 10(3) corresponding tumor cells. Spleen cells from mice immunized by repeated injections of the pretreated antigen neutralized transplantability of the tumor cells. Specific humoral antibody was also detected. The specific antigens obtained from these cells were similar to each other with respect to sedimentation coefficient (16S), electrophoretic characteristics and xenogeneic antigenicity, and were free of beta-2 microglobulin, murine leukemia virus major structural proteins such as gp70 or p30 and murine mammary tumor virus proteins such as gp55 and p28.
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PMID:Purification of cell line-specific transplantation antigens from mouse ascites tumor cells. 711 55

Interleukin IL-27, composed of p28 and EBV-induced gene 3 subunits, has diverse functions in enhancing cell-mediated immunity and silencing the immunity. We examined whether forced expression of the p28-linked EBI3 gene in human oesophageal carcinoma cells (Eca109) produced antitumour effects in a T cell-defective condition. Tumour growth of Eca109 cells expressing IL-27 (Eca109/IL-27) was retarded in nude mice compared with parental and vector DNA-transduced tumours and survival of the mice inoculated with Eca109/IL-27 cells was prolonged. Expression of the tumour necrosis factor-alpha, IL-1beta and IL-6 genes was up-regulated in Eca109/IL-27 tumour specimens while the tumours remained small in size but the increased transcription was subsequently down-regulated in enlarged tumours. Spleen cells from mice-bearing Eca109/IL-27 tumours produced interferon-gamma and showed YAC-1-targeted cytotoxic activities greater than those of mice inoculated with parental or vector DNA-transducer tumours. Numbers of DX5+CD69+ natural killer cells in spleen of mice-bearing Eca109/IL-27 tumours and those of CD31+ cells within Eca109/IL-27 tumours remained the same as found in mice-bearing parental or vector DNA-transduced tumours. These data collectively suggest that the IL-27-mediated antitumour effects produced in a mature T cell-defective condition were attributable to enhanced interferon-gamma production and natural killer activities.
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PMID:IL-27-mediated activation of natural killer cells and inflammation produced antitumour effects for human oesophageal carcinoma cells. 1848 9