Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
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Enzyme
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Query: UMLS:C0151744 (
myocardial ischemia
)
31,282
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Stroke is one of the leading causes of death and long-term disability around the world. Angiogenesis is supposed to protect brain microvascular endothelial cells (BMECs) from oxidative and ischemic stress. Previous studies indicated that interaction between metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and
miR-145
was involved in
myocardial ischemia
reperfusion, suggesting MALAT1 and
miR-145
were also mediated with the progress of angiogenesis and cell migration in oxygen-glucose deprivation (OGD)-induced BMECs. The present study aimed to investigate the functional roles of MALAT1 in regulating
miR-145
and its downstream pro-angiogenesis factors, vascular endothelial growth factor (VEGF)-A and
Angiopoietin-2
(
ANGPT2
) during the progress of angiogenesis in OGD-induced BMECs. An
in vitro
OGD model was employed in mouse BMECs to mimic brain hypoxic and ischemic conditions; MTT was used to determine cell viability. qRT-PCR was used to determine the expression of long non-coding RNA (lncRNA)-MALAT1 and
miR-145
under OGD conditions;
in vitro
tube formation assay was used to investigate angiogenic effect of MALAT1 and
miR-145
The relationship between lncRNA-MALAT1/
miR-145
and
miR-145
/VEGF-A/
ANGPT2
was evaluated by qRT-PCR and Western blot, and direct binding was assessed using dual luciferase assay. Results showed that the levels of lncRNA-MALAT1 and
miR-145
were up-regulated in OGD-induced BMECs.
miR-145
functioned as an anti-angiogenic and pro-apoptotic factor in OGD treated BMECs via down-regulating VEGF-A and
ANGPT2
directly. While lncRNA-MALAT1 enhanced the expressions of VEGF-A and
ANGPT2
by targetting
miR-145
to promote angiogenesis and proliferation of BMECs under OGD conditions. Our present study revealed the inhibitory functions of
miR-145
on angiogenesis through direct targetting on VEGF-A and
ANGPT2
for the first time and proved the protective role of lncRNA-MALAT1 for BMECs under OGD conditions through the direct regulation of
miR-145
.
...
PMID:LncRNA MALAT1 up-regulates VEGF-A and ANGPT2 to promote angiogenesis in brain microvascular endothelial cells against oxygen-glucose deprivation via targetting
miR-145
. 3269 Oct 71
