Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0151744 (myocardial ischemia)
 31,282 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Several workers have used histochemical, enzymatic, and fluorescent methods to diagnose early myocardial ischemia, but the problem of unequivocal detection of early ischemia still remains an enigma to pathologists. In the present study, the left coronary artery was ligated in an animal model, rat, in order to produce myocardial ischemia at different time intervals, from five minutes to six hours. Fluorescent techniques and tetrazolium staining of myocardial succinic dehydrogenases have been used to detect onset of ischemia with the purpose of identifying a sensitive technique for use in routine pathologic specimens. Nitroso-blue tetrazolium and triphenyl tetrazolium chloride staining of myocardium showed loss of dehydrogenases within five to twenty minutes of ligation of the coronary artery. This loss was consistent and progressively increased at longer time intervals, the mean ischemic area mapped being 25.74 mm2 and 66.87 mm2 at five to twenty minutes and six hours respectively. Such comparison of ischemic area of myocardium at different time intervals has not been reported earlier. Autofluorescence in formalin-fixed, hematoxylin and eosin-stained sections showed positive fluorescence only after fifty to seventy-five minutes of ischemia and was patchy in distribution in the left ventricular wall even up to six hours of ligation. Examination of myocardium under fluorescent light after acridine orange staining proved to be more sensitive than autofluorescence for detecting ischemia. At five to twenty minutes, the mean ischemic area was 18.67 mm2 and by six hours it increased to 27.48 mm2.(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:Histochemical and fluorescent techniques for detection of early myocardial ischemia following experimental coronary artery occlusion: a comparative and quantitative study. 245 Apr 89

Myocardial ischaemia and reperfusion injury in 16 anaesthetized Sprague-Dawley rats, eight of which were pretreated with morphine (5 mg/kg, intraperitoneally) to prevent arrhythmias, were studied immunocytochemically with anti-muscle actin specific monoclonal antibody (HHF35). Eight cases of simple ischaemia and eight cases of sham-operated rats were used as controls. With HHF35 ABC immunocytochemical method, the left ventricular myocardium in the reperfusion group (without morphine) showed large areas of staining loss, but no loss of staining was seen in controls. No significant changes were seen with H&E stain in all hearts. Compared with HBFP, acridine orange (AO), eosin-fluorescence, HHF35 ABC staining best demonstrated myocardial reperfusion injury. The results indicate that the degree of myocardial damage may be related to the arrhythmias.
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PMID:Immunocytochemical study with anti-muscle actin antibody (HHF35) on myocardial ischaemia and reperfusion injury in rats. 768 97

Derangement in freeradical processes (FRP) and lipid metabolism is known to occur in ischemic heart disease. Since autoimmune component appear to play an important role in the pathogenesis of IHD, the contributers to the article studied the state of freeradical processes in IHD patients during development of autosensibilization. A total of 42 patients with unstable angina and 8 essentially healthy subjects were examined. The blood level of FRP, antioxidant protection were determined using chemiluminometry technique. The blood serum content of free cholesterol and phospholipid fatty acid composition were evaluated by gas-liquid chromatography, the T- and B-lymphocyte functional status by fluorescent probing with acridine orange. The level of autoantibodies was determined as recommended by Boiden. The lymphocyte circulating population functional activity was found to be related to the state of FRP, antioxidant protection and the blood serum lipidic complex composition.
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PMID:[The role of free radical processes in the development of autosensitization in ischemic heart disease]. 881 21