Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: UMLS:C0151744 (
myocardial ischemia
)
31,282
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Ischemic heart disease
(
IHD
) is a significant cause of cardiovascular diseases. MicroRNAs (miRNAs) have been thought to be critical regulators in the heart diseases. The present study was aimed to investigate the effect of miR-129-5p on the autophagy and apoptosis by targeting
ATG14
as well as how miR-129-5p worked through the PI3K/AKT/mTOR signaling pathway in H
2
O
2
-induced H9c2 cells. H9c2 cells were induced by H
2
O
2
, after which the expression of miR-129-5p was decreased. Reverse transcription-quantitative polymerase chain reaction (qRT-PCR) was performed to detect the expression level of miR-129-5p in H9c2 cells. In addition, the expression of miR-129-5p and
ATG14
were overexpressed or down-regulated after transfection. The transfection efficiency was verified by qRT-PCR. Cell viability, cell apoptosis, and the expression of autophagy and apoptosis-related proteins were determined by CCK-8, flow cytometry and western blotting, respectively. Furthermore, GFP fusion protein analysis was used to detect the expression level of LC3II which was related to autophagy. As a result, cell viability was decreased and cell autophagy was increased in H
2
O
2
-induced H9c2 cells. MiR-129-5p overexpression inhibited cell injury caused by H
2
O
2
in H9c2 cells which was certified by the increased cell viability and decreased cell autophagy and apoptosis. In addition,
ATG14
was demonstrated to be a target of miR-129-5p which inhibited cell injury by down-regulation of
ATG14
. Moreover, phosphorylation of PI3K/AKT/mTOR pathway was activated by miR-129-5p overexpression or
ATG14
inhibition to alleviate the autophagy and apoptosis in H
2
O
2
-induced H9c2 cells. In conclusion, this study indicated that miR-129-5p inhibited autophagy and apoptosis in H
2
O
2
-induced H9c2 cells partly by down-regulation of
ATG14
through the activation of PI3K/AKT/mTOR pathway.
...
PMID:MiR-129-5p inhibits autophagy and apoptosis of H9c2 cells induced by hydrogen peroxide via the PI3K/AKT/mTOR signaling pathway by targeting ATG14. 3034 24
