Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0149958 (complex partial seizures)
 2,563 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

To assess the contribution of ictal SPECT to the definition of the epileptogenic zone (EZ) prior to surgery in focal drug-resistant epilepsies, we investigated the effect of the timing of injection and seizure semiology on patterns of perfusion and cerebral blood flow changes (CBF) beyond the EZ. In the rat model of amygdala-kindled seizures, we measured CBF changes with the quantitative [(14)C]-iodoantipyrine autoradiographic method during secondary generalized (SGS, n=26 fully-kindled rats) and focal seizures (FS, n=19 partially kindled rats), according to sequential timing of injection with respect to seizure onset. During SGS, the correct lateralization and rough localization of the focus within limbic structures was only possible at the early ictal and post-ictal times, in between we observed widespread rCBF increases. The switch from hyper to hypoperfusion occurred at the time of late ictal injection. The accurate localization of the EZ was obtained in the study of the more subtle FS (stage 0). At stage 1 of the kindling, there was already a remote widespread spreading of hyperperfusion. In patients surgically cured from a mesio-temporal lobe epilepsy (mean post-operative follow-up: 66 months), we retrospectively studied 26 pairs of ictal and interictal pre-operative SPECTs, classified in 3 groups according to the progression of ictal semiology. Using visual analysis of subtracted SPECTs (SISCOM) and group comparisons with a control group (using SPM), we observed more widespread combined hyper and hypoperfusion with the increasing complexity of seizures. In simple partial seizures, the SISCOM analysis allowed a correct localization of the focus in 4/8 patients, whereas the SPM analysis failed to detect significant changes, due to individual variation, spatial normalization and small magnitude of CBF changes. In complex partial seizures with automatisms, SISCOM and SPM analysis showed antero-mesial temporal hyperperfusion (overlapping the EZ), extending to the insula, basal ganglia, and thalamus in the group of patients having dystonic posturing (DP group) in addition to automatisms. Ictal hypoperfusion involved pre-frontal and parietal regions, the anterior and posterior cingulate gyri, to a greater extent in the DP group. In both human and animals studies, we observed a correlation between the extent of composite patterns of hyper/hypoperfusion and the severity of seizures, and the recruitment of remote sub-cortical structures. Hypoperfused areas belong to neural networks involved in perceptual decision making and motor planning, whose transient disruption could support purposeless actions, i.e. motor automatisms.
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PMID:[Epileptogenic and non-epileptogenic zones: blood flow studies of temporo-limbic seizures]. 1835 65

Pullulanase (EC 3.2.1.41) from Bacillus acidopullulyticus could preferentially remove galactose residues from guar galactomannan (GG), a heteropolymer consisting of polymannan backbone with alternating galactose as single-residue side-chain stubs. The enzyme showed maximum activity at pH 4.5 and 45 degrees C and exhibited Michaelis-Menten kinetics with a K(m) and V(max) of 6.0 mg mL(-1) and 1820 nmol min(-1) mg(-1), respectively. The native and modified GG had a viscosity of 4800 and 3800 cps, respectively. The molecular mass of the enzyme-treated GG varied between 152 +/- 0.5 kDa, and its GLC analysis revealed a significant change in galactose-mannose content of 1:3.8, respectively, similar to that of locust bean gum (LBG). FTIR and solid-state CP-MASS (13)C NMR analyses indicated subtle changes in the conformation of modified GG because of the removal of galactose residues. Gel-strength measurements with kappa-carrageenan showed an improvement in the gelling behavior, similar to that shown by LBG. Debranching of GG by pullulanase is an alternative and inexpensive route to produce modified GG with enhanced functional properties, as a cost-effective replacement for LBG.
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PMID:Nonspecific activity of Bacillus acidopullulyticus pullulanase on debranching of guar galactomannan. 1894 37