Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0149925 (small cell lung cancer)
 6,491 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Serum creatine kinase BB (CK-BB) and neuron specific enolase (NSE) were measured with chromatography-fluorometric method and ABC-ELISA in 20 patients with small cell lung cancer (SCLC), 30 patients with non small cell lung cancer (NSCLC), 25 patients with benign pulmonary diseases (BPD) and 30 healthy subjects (C). The results revealed that serum concentrations of CK-BB and NSE in SCLC were significantly greater than those in other three groups (P < 0.001). The mean values of CK-BB and NSE in SCLC, NSCLC, BPD, C were 30.2, 8.4, 6.3, 4.3 IU/L and 52.2, 12.7, 10.3, 9.2 ng/ml, respectively. If values above 9.5 IU/L (CK-BB) and 20.8 ng/ml (NSE) were considered abnormal, 70% and 80% of the values of CK-BB and NSE in SCLC were positive, respectively, which were higher than those in NSCLC (P < 0.005). The serum levels of CK-BB had linear correlation with those of NSE in SCLC (r = 0.7934, P < 0.001). By combined determination, the sensitivity and specificity were increased to 90% and 94% respectively. Therefore, the results suggest that serum CK-BB and NSE be diagnostic markers for SCLC. Concurrent determination may raise their value in clinical use.
 ...
PMID:[Clinical value of measurement of serum creatine kinase BB and neuron specific enolase for small cell lung cancer]. 765 49

The clinical value of the serum biomarker carcinoembryonic antigen (CEA) was evaluated prospectively in 118 patients with small cell lung cancer (SCLC) entered chemotherapy protocol between 1986 and 1992. Five quantitative categories were determined: less than 2.5 ng/ml and 2.6-5.0 ng/ml (the standard normal), 5.1-20.0 ng/ml, 20.1-100 ng/ml and greater than 100 ng/ml. 70% of patients had levels less than 5 ng/ml and only 19% had levels greater than 20 ng/ml. There was no clearcut relationship of plasma CEA level to stage of disease, in which 61% of patients with extensive disease (59 patients) had levels less than 5 ng/ml and 22% of patients with limited disease (59 patients) had levels greater than 5 ng/ml. There was a modest relationship of CEA levels to presence of metastases, in that 50% of patients with metastases had levels greater than 20 ng/ml. The average survival for the pathologic and normal category was almost similar, ranging from 13.27 to 16.81 months. The correlation between disease extent and survival was more sensitive for lactate dehydrogenase (LDH) than for CEA. So CEA as a tumor marker for SCLC must be applied in conjunction with other biomarkers, particularly LDH and neuron specific enolase (NSE) and is meaningful in only a small proportion of patients.
 ...
PMID:[The importance of measuring plasma carcinoembryonic antigen in small-cell anaplastic carcinoma]. 802 51

A 60-year-old male with a small cell carcinoma of the right lower ureter is presented. The tumor mainly comprised a small cell carcinoma but also included a full variety of histological types such as transitional cell carcinoma, squamous cell carcinoma, adenocarcinoma, leiomyosarcoma and chondrosarcoma. Immunohistochemical staining was positive for neuron specific enolase and cluster 1 small cell lung cancer antigen/N-CAM in the small cell carcinoma and S-100 in the chondrosarcoma component. The patient underwent a right nephroureterectomy, and received prophylactic radiation of the pelvic and para-aortic lymph node regions and cisplatin and etoposide combination chemotherapy. Eight months after the chemotherapy, a transitional cell carcinoma was found in the bladder neck, and a cystectomy with urethrectomy performed. To our knowledge, this is the second report of a small cell carcinoma originating from the ureter.
 ...
PMID:A ureteral small cell carcinoma mixed with malignant mesodermal and ectodermal elements: a clinicopathological, morphological and immunohistochemical study. 823 Jul 59

The human placental form of glutathione S-transferase pi (GST-pi) was measured by a sandwich enzyme-linked immunosorbent assay in lung cancer cell lines established in our laboratories. In classic-type small cell lung cancer (SCLC), variant-type SCLC and non-small cell lung cancer (NSCLC), the respective mean GST-pi values were 0.83 +/- 0.88, 3.27 +/- 2.85 and 2.40 +/- 0.76 micrograms/mg protein. Cell lines with high GST-pi content had low levels of neuron specific enolase, which is known as a representative tumor marker for SCLC. This suggests that GST-pi may also be used as a potential marker for NSCLC. The lines with low GST-pi content were more sensitive to radiation than those with high GST-pi content. Cell lines not subjected to prior therapy also showed a good correlation between GST-pi levels and chemosensitivity to cisplatin. The findings suggest that GST-pi can be used as an adjunctive marker for lung cancer.
 ...
PMID:Glutathione S-transferase pi levels in a panel of lung cancer cell lines and its relation to chemo-radiosensitivity. 838 71

Levels of the tumour markers neurone specific enolase (NSE), lactate dehydrogenase (LDH), chromogranin A (ChrA) and carcinoembryonic antigen (CEA) were measured in serum taken at presentation and during treatment, remission and relapse from 154 patients who received chemotherapy for small cell lung cancer at a single centre over a 6 year period. At presentation NSE was the most frequently elevated marker, being raised in 81% of patients and significantly higher in extensive as opposed to limited disease, as were LDH and ChrA. The response rate to therapy was best correlated with presentation level of ChrA, being 79% for those whose levels were within twice the upper limit of normal and 51% above (P < 0.01). Multivariate regression analysis showed NSE, performance status and albumin at presentation to be the best independent predictors of survival. Patients with NSE below twice the upper limit of normal, Karnofsky performance status of 80 or above and albumin 35 g l-1 or above had a median survival of 15 months with 25% alive at 2 years, whilst those with NSE above twice normal, Karnofsky below 80 and albumin less that 35 g l-1 had all died by 8 months. Changes in marker levels during therapy were of low predictive value for outcome although the finding of rising NSE during chemotherapy after an initial fall correlated with significantly reduced duration of remission. There was a strong inverse correlation between the NSE level at the time of response and duration of remission (P < 0.0001). Prediction of relapse was most reliable with ChrA, 52% of patients having rising levels before clinical evidence of disease recurrence.
 ...
PMID:Tumour markers for prediction of survival and monitoring of remission in small cell lung cancer. 838 78

We describe, for the first time, the development of a technique for a long-term selective culture of endocrine (PE) cells from the lungs of normal animals. Epithelial cells were isolated from 1-day-old hamster lungs through mechanical and enzymatic dissociation with collagenase type II. Cells were then cultured in HITES medium which contained RPMI 1640, hydrocortisone, insulin, transferrin, estradiol, sodium selenite, and supplemented with 5% fetal bovine serum (FBS), or medium which contained HITES medium supplemented with bovine serum albumin, phosphoethanolamine, arginine vasopressin, bombesin, and 2% FBS (9N). HITES medium, originally developed for establishment and long-term culture of human small cell lung cancer (SCLC) cell lines, allowed propagation of normal hamster PE cells up to 12 months as a mixed floating-attached cell culture. No difference was noted in the results using HITES or 9N. By 3 months, 80% of the cultured cells contained characteristic dense-core (endocrine type) granules. The cultured PE cells also expressed creatine kinase brain isoenzyme, and general NE markers including neuron specific enolase, and amine handling enzyme activity within the range of SCLC cell lines. Moreover, cultured PE cells contained and secreted immunoreactive calcitonin (iCT) which had a molecular profile similar to that of intact hamster lung. This long-term culture technique should markedly assist in elucidating the role of PE cells in health and disease.
 ...
PMID:Long-term selective culture of hamster pulmonary endocrine cells. 838 31

The aim of this study was to assess a prognostic value of serum specific enolase (NSE) measurement in lung cancer patients. Total number of 105 patients entered the study, including 36 patients with small cell carcinoma and 69 patients with non small cell carcinoma (21-squamous cell carcinoma, 32-adenocarcinoma, 14-large cell carcinoma). Elevated NSE level was observed in 47 (44.8%) patients: in 75% of SCLC patients and 29% of NSCLC patients (p < 0.001). Median survival in NSCLC patients with elevated NSE levels was 27 weeks and in those with normal values-59 weeks. The probability of one year survival in both groups was 22% and 45% respectively (p = 0.27). Median survival in SCLC patients with elevated NSE test was 30 weeks and in those normal levels-61 weeks and the probability of one years survival in both groups was 26% and 62%, respectively (p = 0.34).
 ...
PMID:[Prognostic value of neuron enolase levels in serum of patients with lung neoplasms]. 852 May 60

To evaluate the pronostic value of an elevated seric carcinoembryonic antigen (CEA > 10 ng/ml) at diagnosis, in patients with lung cancer, a pair study was done: couples of patients with same staging and histologic type were established, one patient with high CEA level compared to one patient with normal CEA level (< 5.5 ng/ml). Other markers were measured: neuron specific enolase (NSE), squamous cell carcinoma (SCC) or Cyfra 21-1. Survival was the end point of comparison. For 89 couples created, patients with low CEA level had a better survival rate at one year ( p = 0.02), this prognosis advantage was confirmed by a comparison of survival curves with Mantel-Cox and Breslow test (p = 0.01), but not by the signs test. These differences were also observed for the 71 couples of squamous cell carcinomas and adenocarcinomas, and the apparied signs test was still not significant. The poor prognosis persisted for patients with high CEA level, when one another marker's level (NSE or SCC or Cyfra 21-1) was increased, in comparison with patients with any marker increased. On 29 couples of all histological subtypes or on the 25 couples of non small cell lung cancer, the signs test and the comparison of survival curves were significant, but not the 1 year survival rate. This study shows that a CEA level greater than 10 ng/ml at diagnosis is a poor pronostic factor in patients with lung carcinoma, independent of the stage of disease and of the histologic type.
 ...
PMID:[Prognostic value of pre-therapeutic levels of carcino-embryonic antigen in primary bronchial carcinoma]. 874 67

Recent reports indicate that Small Cell Lung Cancer (SCLC) responds favorably to chemotherapy, and this response is associated with the expression of neuroendocrine (NE) markers. However, the currently available reports on the expression of NE markers in SCLC and Non-Small Cell Lung Cancer (NSCLC) are highly inconsistent and need to be reevaluated. Therefore, we have undertaken the current study to clearly define the expression of NE markers in SCLC and NSCLC using representative cell lines. We studied the NE markers, chromogranin A, Neuron specific enolase (NSE), Leu-7 and synaptophysin using various immunochemical and molecular biological methods. By employing Western blotting method, we found that both SCLC and NSCLC cells express large amounts of NSE and chromogranin A. The natural killer cell surface associated antigen (Leu-7) was expressed specifically only by SCLC cell lines. This finding was further confirmed by immunofluorescence staining of the SCLC cells. We could not detect any synaptophysin levels in any of the above cell types by Western blotting technique. Therefore, we employed reverse transcription polymerase chain reaction (RT.PCR) to study the expression of synaptophysin mRNA and found that both SCLC and NSCLC cells express it, albeit in different amounts.
 ...
PMID:Expression of neuron-specific enolase, chromogranin A, synaptophysin and Leu-7 in lung cancer cell lines. 970 May 77

A new human cancer cell line was established from a metastatic lesion of a small cell lung carcinoma (SCLC-R1) and maintained in continuous culture with a doubling time of 62 h. The SCLC-R1 line, whose ultrastructural features are presented, showed a diploid DNA content, a translocation involving chromosome 16 [t(16;?)(q24;?)] and noticeable deletions in the FHIT (fragile histidine triad) region in the short arm of chromosome 3 [del(3)(p14)] and in the telomeric region of the short arm of chromosome 12 [del(12)(p13)]. The involvement of 12p in metastatic small cell lung cancer is reported here for the first time. No amplification or rearrangements were evident in the c-myc, L-myc, N-myc, int-2, c-erbB-2, H-ras, K-ras, c-mos, and hst-1 genes by Southern blot analysis. Wild-type p53, RB, K-ras and H-ras genes were evident by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis. The neuron specific enolase (NSE) level was much higher in the cell line's cytosol than in the patient's serum and the cell line also had high expression of chromogranin A and cytokeratin 19. SCLC-R1 cells were sensitive to cisplatin, carboplatin and doxorubicin. The clinical history of the patient from whom the cell line was derived is reported. The characteristics of this new cell line indicate it to be a useful experimental model to investigate lung cancer biology and anticancer drug response.
 ...
PMID:Chromosomal alterations, biological features and in vitro chemosensitivity of SCLC-R1, a new cell line from human metastatic small cell lung carcinoma. 971 81


<< Previous 1 2 3 4 5 6 Next >>