Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0149521 (chronic pancreatitis)
 7,199 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Antigen T (Thomsen-Friedenreich), a precursor of blood group MN antigens, with the determinant of a carbohydrate (Gal-GalNac), antigen Lewisx (Lex), a trisaccharide found on Type 2 blood group oligosaccharide chains, Sialo-Lex, the derivatives: of Lex and Lewisy (Ley), a difucosylated tetrasaccharide have, behaved as the oncodevelopment tumor-associated antigens in human colon. In the present study, using monoclonal antibodies in immunohistochemical method, the expression and distribution of these antigens in pancreatic cancer and normal pancreatic tissue were observed and compared. It was found that monoclonal antibody AH8-258 derived against antigen T, SSEA-1 and FH-4 derived against short and long chain antigens Lex, FH-6 and IB 9 derived against Sialo-Lex antigen preferentially stained most pancreatic cancer tissues but rarely the normal pancreatic tissues. However, monoclonal antibodies AH-6, KH-1 and CC-1, derived against antigen Ley, stained the majority of tissues regardless of being malignant or normal and were not able to differentiate cancer from the normal tissues. Antigens T, Lex, Sialo-Lex and Ley were also expressed in chronic pancreatitis but the chance of monoclonal antibodies staining in these tissues (18 approximately 36%) was markedly lower than the staining in cancer tissues (54 approximately 77%) except Ley. It is suggested that in human pancreas, haptens T, Lex and Sialo-Lex, the oncodevelopmental cancer-associated antigens, are highly specific markers for malignancy and likely helpful in the early diagnosis of pancreatic cancer.
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PMID:[Pancreatic cancer-associated new carbohydrate antigen]. 245 61

L-methionine uptake by the parotid gland and pancreas has been compared in 27 patients using a non-invasive methodology. L-methionine was labelled with 11C, a positron emitter with a short half life produced in a cyclotron. 11C-L-methionine concentration was measured in the parotid glands and in the pancreas by external detection using a positron emission tomographic system. 11C-L-methionine uptake by the parotid glands was 4.3 X 10(-3) +/- 1.9 X 10(-3)% of the injected dose per millilitre of tissue (mean +/- SD) in a group of 11 normal non-alcoholic subjects. The uptake was 3.6 +/- 1.3 (X10(-3) in a group of nine alcoholic subjects without pancreatic disorder and it was 4.9 +/- 1.5 (X10(-3) in a third group of seven patients with chronic pancreatitis. These values did not significantly differ. In contrast median pancreatic uptake of 11C-L-methionine was nil in chronic pancreatitis and was lower than that seen in normal subjects (15.3 X 10(-3)% ml, p less than 0.001) and in alcoholic subjects (11.5 X 10(-3)% ml, p less than 0.002). Thus neutral long chain amino acid transport in the parotid gland appears to be independent of that in the exocrine pancreas in chronic pancreatitis. This absence of relationship between the parotid gland and the pancreas in pancreatic disease is in contradiction with the demonstration made in animals of an interaction between these two glands. These results, however, are in agreement with the conclusions drawn from the data collected from the saliva test used by several authors.
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PMID:Comparison of 11C-L-methionine uptake by the parotid gland and pancreas in chronic pancreatitis studied by positron emission tomography. 660 32

Formation of fatty acid ethyl esters (FAEEs, catalyzed by FAEE synthase) has been implicated in the pathogenesis of chronic pancreatitis. In previous studies, we demonstrated that FAEE synthase, purified from rat liver microsomes, is identical to rat liver carboxylesterase (pI 6.1), and structurally and functionally different than that from pancreas. In this study, we purified and characterized rat pancreatic microsomal FAEE synthase, and determined its relationship with rat pancreatic cholesterol esterase (ChE). Since most of the serine esterases express p-nitrophenyl acetate (PNPA)-hydrolyzing activity as well as synthetic activity to form fatty acid esters or amides with a wide spectrum of alcohols and amines, respectively, we used PNPA-hydrolyzing activity to monitor the purification of FAEE synthase during various chromatographic purification steps. Synthesizing activity towards FAEEs, fatty acid methyl esters, and fatty acid anilides was measured only in the pooled fractions. At each step of purification (ammonium sulfate saturation, Q Sepharose XL, and heparin-agarose column chromatographies, and high performance liquid chromatography (anion exchange and gel filtration)) synthetic as well as hydrolytic activities copurified. Using ethanol, methanol, or aniline as substrates, the ester or anilide synthesizing activity of the purified protein was found to be 8709, 13000, and 2201 nmol/h/mg protein, respectively. The purified protein displayed a single band with an estimated molecular mass of approximately 68 kD upon SDS-PAGE under reduced denaturing conditions, cross-reacted with antisera against rat pancreatic ChE and showed 100% N-terminal sequence homology of the first 15 amino acids to that of rat pancreatic ChE. These results suggest that the purified protein has broad substrate specificity towards the conjugation of endogenous long chain fatty acids with substrates having hydroxyl and amino groups and is identical to ChE.
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PMID:Purification and characterization of rat pancreatic fatty acid ethyl ester synthase and its structural and functional relationship to pancreatic cholesterol esterase. 1470 89