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Query: UMLS:C0085631 (agitation)
 12,064 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Granulocyte and lymphocyte suspensions were exposed to slight mechanical agitation for 30 s on a tube mixer before being examined for membrane receptors by rosette techniques. Agitation caused an increase in the proportion of granulocytes bearing receptors for sheep erythrocytes (E-R), for the Fc portion of IgG (Fc gamma-R), and for C3b (C3b-R), and of lymphocytes bearing the Fc gamma-R. The proportion of E-R-bearing lymphocytes was not enhanced, with the exception of the 'early' or 'active' E-R-bearing cells. The increase in rosette formation was specific, i.e. rosette formation with ox or rabbit E was not induced by agitation. The increased proportion of membrane receptors was not associated with an increase in granulocyte phagocytosis of preopsonized zymosan particles, as measured by chemiluminescence. Membrane perturbation thus probably increases the availability of surface receptors.
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PMID:Enhancement of leukocyte membrane receptor expression after mechanical agitation. 294 89

The sizes of the alveolar macrophage (AM) and interstitial macrophage (IM) populations in the lungs of adult Fischer-344 rats were determined during steady state. AM labeled with opsonized erythrocytes during an in situ phagocytic assay were lavaged from excised lungs. The lungs were then dispersed into single-cell suspensions with collagenase and mechanical agitation, and the remaining mononuclear phagocytes were identified following a second labeling step. The size of the AM population was 1.3 X 10(7) cells, or approximately equal to 3% of the total lung cell population. The AM were negative for cytoplasmic myeloperoxidase granules. The size of the IM population was 8 X 10(6) cells, or approximately equal to 2% of the total lung cell population. IM were also negative for myeloperoxidase and, like AM, demonstrated marked Fc gamma R-mediated phagocytic activity. The high cell yields (approximately equal to 4 X 10(8) cells/lung; viability, greater than 85%) and the percentages of type II cells (11%) and ciliated epithelial cells (less than 0.5%) indicated the enzymatic dispersion method resulted in a highly efficient and representative sampling of the lung parenchyma. The collagenase method used in this study to disperse the lung cells into single-cell suspensions, in conjunction with additional cell separation techniques, may be of potential use for isolating enriched populations of IM, as well as other lung cell types, for in vitro study.
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PMID:Pulmonary macrophages: alveolar and interstitial populations. 300 Jul 57