Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0085631 (agitation)
 12,064 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

With the use of monoclonal antibodies against the CD34 antigen, flow cytometry (FC) permits rapid assessment of the quality of hematopoietic grafts. We examined PBSC samples (n = 40) to investigate possible influences of storage time and temperature on FC results. After cytapheresis, a sample of the PBSC product was collected and divided into 4 aliquots. Immediate analysis was performed on one aliquot. The other 3 specimen were stored for a) 24 h at room temperature (RT, 20 +/- 2 degrees C), at room temperature with agitation and c) at +4 degrees C. For flow cytometric analyses, samples were labeled with two CD34 markers (HPCA-2, Becton Dickinson Corp. [BD], USA; QBend-10, Immunotech Corp. [IT], Germany). After 24 h CD34+ signals had decreased by 25.4% (BD) and 27.0% (IT) in average, when samples were stored at room temperature in comparison to the results obtained directly after cytapheresis (p < 0.05). At RT in combination with agitation, there was an increase in signal rates compared to baseline values, probably due to binding of CD34 antibodies to myeloid or non-viable cells (+ 9.2% [BD] and 11.2% [IT]). At a storage temperature of +4 degrees C, CD34+ events did not decrease significantly (-0.7% [BD] and -0.2% [IT]). Our data demonstrate that FC results may be influenced by temperature, agitation and storage time.
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PMID:Storage of peripheral blood stem cell samples alters flow cytometric CD34+ results. 886 43