Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0085631 (agitation)
 12,064 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In a 5-L fermentor (NBS-MF 105), Saccharomyces cerevisiae W303-181 (1.0 g dry matter/L) was inoculated into 3.0 L of liquid medium containing glucose (10 or 20 g/L), yeast nitrogen base (YNB, 3.7 or 7.4 g/L), l-histidine (0.02 g/L), l-tryptophan (0.02 g/L), uracil (0.02 g/L), and adenine (0.02 g/L). The culture was carried out batchwise for 12 or 24 h at 30 degrees C, pH 4.6 or 5.7, aeration of 0, 0.8, 1.7 or 2.2 vvm, and agitation of 400 rpm. The highest G6PDH productivity (10.5 U/L.h) and specific activity (320 U/mg of protein) occurred at aeration of 2.2 vvm, pH 5.7, 10 g/L of glucose, and 3.7 g/L of YNB. The G6PDH specific activity attained was comparable with those of commercial preparations, which are between 50 and 600 U/mg of protein.
 ...
PMID:Production of glucose 6-phosphate dehydrogenase from genetically modified Saccharomyces cerevisiae grown by batch fermentation process. 1608 Jun 93

Alkaline lipase production was performed in submerged fermentation by Penicillium expansum PED-03. It was found that the suitable carbon source and nitrogen source for lipase production were 0.5% starch and 4.0% soybean meal, respectively. The maximal lipase activity (850 U/mL) of production was achieved at initial pH 5.5-6.0, 26 degrees C, 72 h. Tween-80 was an effective enhancer for lipase production. Agitation speed of the fermentor played an important role, and the suitable agitation speed for lipase production was 500 r/min. The lipase was stable within the range of pH 7.0-10.0 and 20-40 degrees C, and the optimum conditions for the enzymatic reaction were 35 degrees C and pH 9.5. The enzymatic resolution of racemic allethrolone (4-hydroxy-3-methyl-2-(2-propenyl)-2- cyclopenten-1-one) was carried out by the lipase from P. expansum PED-03, and the conversion reached 48% with excellent enantioselectivity (E > 100), which showed a good application potential in the production of optically pure allethrolone.
 ...
PMID:Enhanced production of Penicillium expansum PED-03 lipase through control of culture conditions and application of the crude enzyme in kinetic resolution of racemic Allethrolone. 1608 Jun 97

The effects of nitrogen source on arachidonic acid (AA) production and morphological changes during the culture of Mortierella alpina were investigated using an image analysis system. When yeast extract, gluten meal, or corn steep liquor was used, a circular pellet morphology was obtained. However, when Pharmamedia, fish meal, or soybean meal was used, M. alpina formed radial filamentous mycelia. The radial filamentous area in the case of soybean meal was 75% of the total mycelial area. In a jar fermentor culture, M. alpina morphology varied with the cultivation period: (i) at 0-6 h culture, the inoculated pellet-like mycelia were adapted to the new environment, (ii) at 6 h-1 d culture, filamentous mycelia grew exponentially which yielded a feather-like morphology, (iii) at 1-2 d culture, the filamentous mycelia became disentangled as a result of the mechanical agitation; consequently, the proportion of filamentous mycelia was increased, (iv) at 2-4 d culture, mycelia showed stationary growth, but the AA concentration increased rapidly, and (v) at 4-6 d culture, hyphae grew thick radially with the AA concentration continuing to increase gradually. In the case of the cultures with feather-like morphology obtained using soybean meal, the AA yield was 0.14 g/g dry cell weight, which was two times higher than that in cultures grown using yeast extract. These results suggest that the feather-like morphology of culture of M. alpina is suitable for AA production.
 ...
PMID:Effect of nitrogen source on mycelial morphology and arachidonic acid production in cultures of Mortierella alpina. 1623 75

Tempe is a soybean food obtained by stationary solid-substrate fermentation using moulds (mainly Rhizopus spp.) as starter organism. Traditionally, tempe is fermented in static layer trays or wrapped packages. Due to heat and mass transfer limitations, gradients of temperature and gas atmosphere will result. Agitated fermentation can help to level heat and mass gradients, yielding better homogeneity. This type of process will not result in traditional tempe, but in individually fermented soybeans that could be processed into food ingredients. This report deals with the comparative effect of stationary versus agitated solid-substrate fermentation of soybeans on some chemical indices of substrate modification. For agitated solid-substrate fermentation, a 450-l size rotary-drum bioreactor was designed and constructed. Of two Rhizopus spp. tested, R. microsporus tolerated agitation quite well, as judged by changes of pH, amino nitrogen, ammonia, and soluble dry matter. The other species, R. oligosporus was strongly affected by agitation. This resulted in less pH increase (difference approx. 1.5 units), lower amino nitrogen levels (30-50% of levels in static fermentation), and lower levels of water-soluble non-lipid dry matter (30-50% of levels in static fermentation) with R. oligosporus agitated fermentation of soybeans controlled at 30 and 37 degrees C, compared to static fermentation at temperatures ranging between 25-35 and 30-40 degrees C, respectively.
 ...
PMID:Solid-substrate fermentation of soybeans with Rhizopus spp.: comparison of discontinuous rotation with stationary bed fermentation. 1623 99

The regulation of an H(2)O(2)-dependent ligninolytic activity was examined in the wood decay fungus Phanerochaete chrysosporium. The ligninase appears in cultures upon limitation for nitrogen or carbohydrate and is suppressed by excess nutrients, by cycloheximide, or by culture agitation. Activity is increased by idiophasic exposure of cultures to 100% O(2). Elevated levels of ligninase and, in some cases, of extracellular H(2)O(2) production are detected after brief incubation of cultures with lignins or lignin substructure models, with the secondary metabolite veratryl alcohol, or with other related compounds. It is concluded that lignin degradation (lignin --> CO(2)) by this organism is regulated in part at the level of the ligninase, which is apparently inducible by its substrates or their degradation products.
 ...
PMID:Factors Involved in the Regulation of a Ligninase Activity in Phanerochaete chrysosporium. 1634 16

A new class of materials based on inorganic and organic species combined at a nanoscale level has received large attention recently. In this work the idea of producing hybrid materials with controllable properties is applied to obtain foams to be used as scaffolds for tissue engineering. Hybrids were synthesized by reacting poly(vinyl alcohol) in acidic solution with tetraethylorthosilicate. The inorganic phase was also modified by incorporating a calcium compound. Hydrated calcium chloride was used as precursor. A surfactant was added and a foam was produced by vigorous agitation, which was cast just before the gel point. Hydrofluoric acid solution was added in order to catalyze the gelation. The foamed hybrids were aged at 40 degrees C and vacuum dried at 40 degrees C. The hybrid foams were analyzed by Scanning Electron Microscopy, Mercury Porosimetry, Nitrogen Adsorption, X-ray Diffraction and Infra-red Spectroscopy. The mechanical behavior was evaluated by compression tests. The foams obtained had a high porosity varying from 60 to 90% and the macropore diameter ranged from 30 to 500 microm. The modal macropore diameter varied with the inorganic phase composition and with the polymer content in the hybrid. The surface area and mesopore volume decreased as polymer concentration increased in the hybrids. The strain at fracture of the hybrid foams was substantially greater than pure gel-glass foams.
 ...
PMID:Preparation of bioactive glass-polyvinyl alcohol hybrid foams by the sol-gel method. 1638 85

Antrodia cinnamomea is a medicinal fungus that has been used in Taiwan as a traditional medicine for the treatment of tumorigenic diseases. We prove that controlling the culturing conditions (i.e., temperature and pH) and modifying the composition of the medium (i.e., carbon, nitrogen, mineral sources and vitamins) can dramatically enhance the production of the exopolysaccharide of A. cinnamomea. We have found that the temperature, initial pH, and agitation time are all critical for exopolysaccharide production during the cultivation of A. cinnamomea in submerged cultures; our optimized conditions were 28 degrees C, pH 5.5, and 14 days, respectively. In addition, when optimizing the effects of additional nutrition, we found that 5% (v/v) glucose, 0.5% (v/v) calcium nitrate, 0.1% (v/v) ferrous sulfate, and 0.1% (v/v) nicotinic acid led to the greatest production of exopolysaccharides; the exopolysaccharide production, mycelial biomass and specific product yield reached 0.49 g/l, 2.60 g/l and 0.19 g/g, respectively. The results indicate that nutrients can be utilized to improve the production of exopolysaccharide and that good mycelial growth does not seem to be a determining factor for a high production yield of exopolysaccharide in A. cinnamomea.
 ...
PMID:Cultivating conditions influence exopolysaccharide production by the edible Basidiomycete Antrodia cinnamomea in submerged culture. 1643 17

The diversity and content of available nitrogen sources in the growth medium both are very important in the accumulation of ergosterol in the yeast cell membrane. Growth on the good nitrogen sources such as ammonia can harvest more yeast cells than on poor ones, but ergosterol content in those yeast cells is relatively lower. Ergosterol content, one of the most variable parameters in ergosterol production by yeast cultivation, is greatly influenced by nitrogen limitation. The aim of our work was to study how the nitrogen sources affected the membrane ergosterol content and increase the total ergosterol yield. On the premise of keeping high ergosterol content in yeast cell, the ergosterol yield was enhanced by increasing the yeast biomass. Direct feed back control of glucose using an on-line ethanol concentration monitor was introduced to achieve high cell density. Ammonia, which acted as nitrogen source, was added to adjust pH during fermentation process, but its addition needed careful control. Cultivation in 5 L bioreactor was carried out under following conditions: culture temperature 30+/-1 degrees C, pH 5.5+/-0.1, agitation speed 600 rpm, controlling ethanol concentration below 1% and controlling ammonium ion concentration below 0.1 mol/L. Under these conditions the yeast dry weight reached 95.0+/-2.6 g/L and the ergosterol yield reached 1981+/-34 mg/L.
 ...
PMID:Effect of nitrogen limitation on the ergosterol production by fed-batch culture of Saccharomyces cerevisiae. 1648 99

This study was conducted to examine the effect of a quick-freezing protocol on morphological survival and in vitro development of mouse embryos cryopreserved in ethylene glycol (EG) at different preimplantation stages. One-cell embryos were harvested from 6-to 8-wk-old CB6F1 superovulated mice, 20 to 23 h after pairing with males of the same strain and hCG injection. The embryos were cultured in human tubal fluid (HTF) containing 4 mg/ml BSA under mineral oil at 37 degrees C in 5% CO(2) plus 95% room air at maximal humidity. Twenty-four to 96 h after collection, the embryos were removed from culture and frozen at the 2 cell, 4 to 8-cell, compact morula, early blastocyst, expanding blastocyst and expanded blastocyst stages. To perform the quick-freeze procedure, embryos were equilibrated in Dulbecco's phosphate buffered saline (DPBS) + 10 % fetal bovine serum (FBS) + 0.25 M sucrose + 3 M ethylene glycol (freeze medium) for 20 min at room temperature (22 to 26 degrees C) and loaded in a single column of freeze medium into 0.25-ml straws (4 to 5 embryos per straw). The straws were held in liquid nitrogen vapor for 2 min and immersed in liquid nitrogen. Embryos were thawed by gentle agitation in a 37 degrees C water bath for 20 sec and transferred to DPBS + 10 % FBS + 0.5 M sucrose (re-hydration medium) for 10 min at room temperature, rinsed 2 times in HTF plus 4 mg/ml BSA and then cultured for 24 to 96 h. Survival of embryos was based on their general morphological appearance after thawing and their ability to continue development upon subsequent culture in vitro. Survival of blastocysts after thawing also required expansion or reexpansion of the blastocoel after several hours in culture. Significant differences were found in the survival and development of mouse embryos at different developmental stages quick-frozen in ethylene glycol and sucrose: 2-cell embryos 43/84 (51%), 4 to 8-cell embryos 44/94 (47%), morulae and early blastocysts 56/70 (80%; P</=0.05), expanding and expanded blastocysts 10/59 (17%; P</=0.05). Our data indicate that the developmental stage in which mouse embryos are subjected to this quick-freeze protocol affects survival and development in vitro and that most (80%) morula and early blastocyst stage embryos survive the procedure.
 ...
PMID:The effect of quick-freezing in ethylene glycol on morphological survival and in vitro development of mouse embryos frozen at different preimplantation stages. 1672 6

Solid phase microextraction (SPME) was used for the extraction of residual coumaphos and dichlorvos in whole milk. The residues were analyzed by capillary gas chromatography equipped with nitrogen phosphorus detector (GC-NPD). A manual SPME holder with a 100-microm polyacrylate fiber was used. The optimized conditions for extraction by SPME method were: sample agitation, absorption temperature of 30 degrees C, absorption time of 40 min, desorption time of 10 min, and sample volume was 16.0 mL in the vial. Under these conditions, the calibration graphs were linear in the range of 0.17 microgL-1 to 1.75 microgL-1 for coumaphos and 0.69 microgL-1 to 6.90 microgL-1 for dichlorvos. Precision was good with RSD values of 13% for coumaphos and 14% for dichlorvos. The detection limits (LOD) were 0.060 microgL-1 for dichlorvos and 0.052 for coumaphos. The quantification limits (LOQ) were 0.086 microgL-1 for dichlorvos and 0.066 microgL-1 for coumaphos. The results obtained in this study suggest that SPME is a suitable technique for residual pesticide analysis of milk. The data demonstrate that particular OP pesticides used in dairy farming in the region of Minas Gerais were found to contaminate cow whole milk, and the residues are not removed by treating the milk by boiling.
 ...
PMID:Analysis of organophosphorus pesticides in whole milk by solid phase microextraction gas chromatography method. 1675 56


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>