Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: UMLS:C0085593 (chills)
 4,268 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Exposure for 20 min of stationary phase cells of Salmonella typhimurium to a combined triple stress system (TSS) treatment comprising hypochlorite derived 5 ppm free available chlorine in solution acidified with 1% succinate (pH 2.5) and at a chill shock temperature of 5 degrees C resulted in symptoms of injury. Cells became sensitive to 40 micrograms/ml lysozyme, 50 micrograms/ml actinomycin D and 100 micrograms/ml ribonuclease B, to which control cells were resistant. Metabolic injury was indicated by reduction in colony forming ability of stressed cells on minimal salts glucose agar M9 medium. There was no detectable leakage loss of 260-280 nm-absorbing materials. This was also confirmed by assay of the cellular RNA material components. Loss of alkaline phosphatase activity was observed in the stressed cells. The intensity of induced cellular damage as measured by lysozyme sensitivity was greatest in the cells exposed to the complete TSS, followed by those stressed in 1% succinate at 5 degrees C, then 5 ppm chlorine at 5 degrees C and the singular chill shock stress at 5 degrees C, respectively. The magnitudes of cellular damage, however, were suggestive of synergistic interactions among the component stress factors of the TSS. The findings obtained indicated impairment of the structural integrity and functional capabilities of the permeability barriers and the inactivation of certain periplasmic enzymes. The resultant cumulative cellular damage from the TSS exposure may therefore enhance greater sensitivity of treated cells to subsequent stress factors.
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PMID:Mechanisms of triple stress-mediated damage in stationary phase cells of Salmonella typhimurium exposed to succinate-acidified hypochlorite system at 5 degrees C. 242

A study was conducted to determine effects of bird age at slaughter, feed withdrawal, and transportation on levels of coliforms, Campylobacter, Escherichia coli, and Salmonella on carcasses before and after immersion chilling. Broilers were processed at 42, 49, and 56 d of age after a 12-h feed withdrawal period or a 0-h feed withdrawal period (full fed). At each age, broilers were processed from two commercial farms previously identified as Campylobacter positive. One week before slaughter, broilers were gavaged with nalidixic acid-resistant Salmonella. During bleeding, cotton plugs were inserted into the cloaca of each carcass. Whole-carcass rinses (WCR) were performed before and after immersion chilling with 20 ppm sodium hypochlorite, and rinses were analyzed for coliforms, Campylobacter, E. coli and Salmonella. Log10 counts for coliforms, Campylobacter, and E. coli were (P < 0.05) affected by bird age at slaughter. Feed withdrawal (FW) affected only Campylobacter on carcasses of older broilers (56 d of age). Chilling with sodium hypochlorite resulted in log10 reductions of 1.2, 1.3, 1.4, and 0.5 for coliforms, Campylobacter, E. coli, and Salmonella, respectively. Under the conditions of this experiment, it appears that contamination on the exterior of birds entering the processing facility is critical to carcass bacterial counts. Moreover, carcass bacterial counts did not vary when microbial counts of broilers were comparable. FW may increase prechill carcass counts for E. coli and Campylobacter, but it appears to have no effect on postchill carcass counts when sodium hypochlorite is used in the chilling operation.
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PMID:Effect of broiler age, feed withdrawal, and transportation on levels of coliforms, Campylobacter, Escherichia coli and Salmonella on carcasses before and after immersion chilling. 1258 Feb 62

There is an increasing need for methods of cryopreservation of arthropods. In particular, Lepidoptera are extremely important in entomological applications for the protection of agricultural crops and forest ecosystems and also in many aspects of biodiversity conservation. Yet, few studies have dealt with cryopreservation techniques in species of this insect order. The aim of this study was to examine the chill sensitivity of eggs of the greater wax moth Galleria mellonella (L.) and the possibility to cryopreserve the eggs by vitrification methods. One day-old eggs were dechlorinated with water solutions of 1.25% sodium hypochlorite and 0.04% Tween 80, treated with cryoprotective agents in two steps, subjected to rapid cooling by immersion in LN and stored in a mechanical freezer for 48 h at -140 degrees C. They exhibited survival rates of 1.6+/-0.5% after being cooled in LN and 0.6+/-0.2% after being stored in the mechanical freezer. 92.9% of the larvae that hatched from cryopreserved eggs completed development regularly, producing adults that bred and laid fertile eggs. The hatching rate of eggs in the F1 and F2 generations was higher than 90%. Adult emergences of the progeny of eggs stored at ultra-low temperatures allowed us to establish a laboratory colony.
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PMID:Chill sensitivity and cryopreservation of eggs of the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae). 1795 Feb 66

One hundred thirty-two pork carcasses were used with 36 of these receiving a wash with tap water and 96 receiving a wash with tap water plus 200 ppm of sodium hypochlorite. The first trial involved 16 carcasses measured for total aerobic psychrotrophs. A reduction (P<.05) in the bacterial level was found on those carcasses sprayed for 10 min with a 200 ppm sodium hypochlorite solution. Three additional trials involved 116 carcasses that were measured for degree of shrinkage after a 24 h chill. Shrinkage was reduced (P<.01) from a high of 2.46% for the controls to slight increases in weight for those treated for 30 min. Generally there was a linear relationship with reduction of shrinkage and length of spraying with a 200 ppm sodium hypochlorite solution.
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PMID:Bacteriology and Weight Loss of Pork Carcasses Treated with a Sodium Hypochlorite Solution 1. 3094 17