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Target Concepts:
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Query: UMLS:C0085110 (
SCID
)
11,041
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We previously identified CD34-negative (CD34
-
)
severe combined immunodeficiency
(
SCID
)-repopulating cells as primitive hematopoietic stem cells (HSCs) in human cord blood. In this study, we develop a prospective ultra-high-resolution purification method by applying two positive markers, CD133 and
GPI-80
. Using this method, we succeed in purifying single long-term repopulating CD34
-
HSCs with self-renewing capability residing at the apex of the human HSC hierarchy from cord blood, as evidenced by a single-cell-initiated serial transplantation analysis. The gene expression profiles of individual CD34
+
and CD34
-
HSCs and a global gene expression analysis demonstrate the unique molecular signature of CD34
-
HSCs. We find that the purified CD34
-
HSCs show a potent megakaryocyte/erythrocyte differentiation potential in vitro and in vivo. Megakaryocyte/erythrocyte progenitors may thus be generated directly via a bypass route from the CD34
-
HSCs. Based on these data, we propose a revised road map for the commitment of human CD34
-
HSCs in cord blood.
...
PMID:A revised road map for the commitment of human cord blood CD34-negative hematopoietic stem cells. 2987 83
Hematopoietic stem cell (HSC) biology is a current topic of interest having significant implications for clinical HSC transplantation and basic HSC research. It was long believed that the most primitive HSCs in mammals, including those in mice and humans, were CD34 antigen-positive (CD34
+
) cells. However, Nakauchi et al. reported that murine long-term lymphohematopoietic reconstituting HSCs were lineage marker-negative (Lin
-
) c-kit
+
Sca-1
+
CD34-low/negative (CD34
low/-
), known as CD34
low/-
KSL cells. We have previously identified very primitive human cord blood (CB) -derived CD34-negative (CD34
-
)
severe combined immunodeficiency
(
SCID
) -repopulating cells (SRCs) utilizing an intra-bone marrow injection method and have proposed a new concept that CD34
-
SRCs (HSCs) reside at the apex of human HSC hierarchy. Recently, we developed an ultra-high-resolution purification method using the two positive/enrichment markers CD133 and
GPI-80
and succeeded in purifying CD34
+/-
HSCs on a single-cell level. On the basis of these data, we propose a revised roadmap for the commitment of human CD34
-
HSCs. This review updates the concept of the stem cell nature of human CB-derived primitive CD34
+/-
HSCs.
...
PMID:[Updated human hematopoietic stem cell findings: purification of human hematopoietic stem cells and elucidation of their hierarchy]. 3030 86
