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Disease
Symptom
Drug
Enzyme
Compound
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Target Concepts:
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Query: UMLS:C0079731 (
B-cell lymphoma
)
16,671
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Tumor suppressor genes are frequently deleted or mutated in lung cancer. The
RNA-binding motif protein 10
(
RBM10
) gene has the ability to suppress tumor activity, but the role of
RBM10
during the development of lung cancer has yet to be elucidated. The current study investigated the expression levels of
RBM10
in non-tumor and tumor tissues obtained from patients with adenocarcinoma using reverse transcription-polymerase chain reaction and western blot analysis, and identified a reduction in
RBM10
expression in lung tumor tissue. To investigate the
in vitro
and
in vivo
function of
RBM10
, A549 human non-small cell lung cancer cells were transfected with the pcDNA-
RBM10
vector. Flow cytometry was used to analyze the levels of apoptosis in the transfected cells. Western blot analysis was used to evaluate the expression of
B-cell lymphoma
2 (Bcl-2), cleaved caspase-3, caspase-9 and poly (ADP-ribose) polymerase (PARP) proteins in A549 cells and tissues from the A549 xenograft Bagg Albino coat (BALB/c) nude mice model.
RBM10
mRNA levels were significantly decreased in adenocarcinoma cells, but not in the non-tumor tissues. The A549 cells and tumor tissues exhibited significant growth inhibition following transfection with the pcDNA-
RBM10
vector, which was determined using a cell proliferation assay. Flow cytometry analysis of cells stained with Annexin V/propidium iodide indicated that the overexpression of
RBM10
induced apoptosis in A549 cells. The present study demonstrated that the expression levels of Bcl-2 protein were decreased and the expression levels of cleaved caspase-3, caspase-9 and PARP proteins were significantly increased in the A549 cells and cells from
ex vivo
tumor tissues that were injected with
RBM10
vector-containing
Salmonella enterica
subspecies enterica serovar typhimurium. Notably, the current study identified that the accumulated and stable overexpression of
RBM10
in the xenograft BALB/c nude mice model significantly inhibited the tumor growth rate. These results may provide novel insights into the use of
RBM10
for lung cancer diagnosis and therapy.
...
PMID:Increased cell apoptosis in human lung adenocarcinoma and
in vivo
tumor growth inhibition by
RBM10
, a tumor suppressor gene. 2908 65
