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Target Concepts:
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Query: UMLS:C0043167 (
pertussis
)
19,595
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Bordetella
pertussis
, the agent of whooping cough, can invade and survive in several types of eukaryotic cell, including CHO, HeLa 229, and HEp-2 cells and macrophages. In this study, we analyzed bacterial invasiveness in nonrespiratory human HeLa epithelial cells and human
HTE
and HAE0 tracheal epithelial cells. Invasion assays and transmission electron microscopy analysis showed that B.
pertussis
strains invaded and survived, without multiplying, in
HTE
or HAE0 cells. This phenomenon was bvg regulated, but invasive properties differed between B.
pertussis
strains and isolates and the B.
pertussis
reference strain. Studies with B.
pertussis
mutant strains demonstrated that filamentous hemagglutinin, the major adhesin, was involved in the invasion of human tracheal epithelial cells by bacteria but not in that of HeLa cells. Fimbriae and
pertussis
toxin were not found to be involved. However, we found that the production of adenylate cyclase-hemolysin prevents the invasion of HeLa and
HTE
cells by B.
pertussis
because an adenylate cyclase-hemolysin-deficient mutant was found to be more invasive than the parental strain. The effect of adenylate cyclase-hemolysin was mediated by an increase in the cyclic AMP concentration in the cells. Pertactin (PRN), an adhesin, significantly inhibited the invasion of
HTE
cells by bacteria, probably via its interaction with adenylate cyclase-hemolysin. Isolates producing different PRNs were taken up similarly, indicating that the differences in the sequences of the PRNs produced by these isolates do not affect invasion. We concluded that filamentous hemagglutinin production favored invasion of human tracheal cells but that adenylate cyclase-hemolysin and PRN production significantly inhibited this process.
...
PMID:Role of adhesins and toxins in invasion of human tracheal epithelial cells by Bordetella pertussis. 1072 85
