Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0043167 (pertussis)
 19,595 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Effects of Gpp(NH)p and pretreatment of membranes with pertussis toxin (PT) were studied on opioid binding to bovine adrenal medullary opioid receptors. Gpp(NH)p inhibited [3H]DADLE binding by increasing the dissociation constant. PT treatment reduced [3H]DADLE binding and almost abolished the Gpp(NH)p inhibition of [3H]DADLE binding. Levorphanol did not show any significant effect on bovine adrenal medullary adenylate cyclase activity. These results suggest that bovine adrenal medullary opioid receptors are linked to PT-sensitive GTP-binding proteins which are not directly coupled to adenylate cyclase.
NIDA Res Monogr 1986
PMID:Adrenal medullary opioid receptors are linked to GTP-binding proteins, pertussis toxin substrates. 282 56

The expression of opioid receptors and GTP-binding proteins was studied in 14 pheochromocytomas. The amounts of [3H]diprenorphine bound to membranes varied from 13 to 62 fmole/mg protein, but significantly higher in adrenaline-secreting tumors than in noradrenaline-secreting tumors. None of [3H]DADLE, [125I]beta-endorphin or [3H]ethylketocyclazocine binding was correlated with [3H]diprenorphine binding. Gpp(NH)p inhibition of [3H]DADLE binding was evident in all four normal human adrenal medullae but in only 8 out of 14 pheochromocytomas. The extent of Gpp(NH)p inhibition was not correlated with the amount of pertussis toxin (PT)-sensitive GTP-binding proteins as measured by PT-catalyzed [32P]ADP-ribosylation. The present findings suggest that opioid receptors and PT-sensitive GTP-binding proteins are variously expressed in transformed chromaffin cells, pheochromocytoma.
NIDA Res Monogr 1986
PMID:Varying expression of opioid receptors and GTP-binding proteins in human pheochromocytomas. 282 58

After treatment of mouse spinal cord-ganglion explants with pertussis toxin (PTX), the acute depressant effects of opioids on sensory-evoked dorsal-horn network responses are markedly attenuated, and characteristic cord discharges can then occur even in the presence of greater than 100-fold higher opioid concentrations, as observed after chronic exposure to opioids. The usual acute depressant effects of serotonin, norepinephrine, and oxotremorine on dorsal-horn discharges are similarly attenuated in PTX-treated cultures. These results together with our previous physiologic and biochemical analyses of adenylate cyclase (AC) and cyclic AMP (cAMP) activities in cord-ganglion cultures suggest that the neuromodulatory effects of opioid, monoaminergic and muscarinic agonists on primary afferent networks in the spinal cord may be mediated by binding to neuronal receptor subtypes that are negatively coupled via Gi to a common pool of AC.
NIDA Res Monogr 1986
PMID:Pertussis toxin treatment results in tolerance to the depressant effects of opioid, monoaminergic, and muscarinic agonists on dorsal-horn network responses in mouse spinal cord-ganglion cultures. 312 34

Autoimmune uveitis is a serious sightthreatening disease that in many cases fails to respond to conventional immunosuppressive or biological therapy. Experimental models used in research allow more detailed study of pathogenesis of the autoimmune process and testing new therapeutic strategies. Recent results show that infection can trigger autoimmune diseases, and some commensal microorganisms are essential in causing disease activity. The aim of this work was to assess the effect of broadspectrum antibiotics - combination of metronidazole and ciprofloxacin or metronidazole alone - on the intensity of intraocular inflammation in experimental autoimmune uveitis (EAU). EAU was induced in mouse strain C57BL/6J by interphotoreceptor retinoid- binding protein in complete Freund's adjuvant and pertussis toxin. The grade of uveitis was assessed clinically and histologically in haematoxylin and eosin- stained tissues. Lymphocytes and macrophages were detected in cryosections using the immunoperoxidase method with antibodies. The therapy was commenced one week before EAU induction and continued throughout the experiment. In addition, metronidazole treatment was also started two weeks before EAU induction. Antibiotics significantly reduced the intensity of uveitis compared to the control group (P < 0.05). The effects of combination of ciprofloxacin and metronidazole and of metronidazole alone were similar when the therapy started one week before EAU induction (P < 0.05). Metronidazole commenced two weeks before EAU induction and throughout the experiment suppressed the intensity of EAU with even higher statistical significance (P < 0.0001). It can be assumed that the high protective effect of metronidazole on EAU intensity may be due not only to its antimicrobial effect, but also to its immunomodulatory activity.
 ...
PMID:Metronidazole Attenuates the Intensity of Inflammation in Experimental Autoimmune Uveitis. 3236 10