Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
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Target Concepts:
Gene/Protein
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Query: UMLS:C0043167 (
pertussis
)
19,595
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Commercially available Amies transport medium with charcoal and three isolation media were tested to assess their efficiency in the clinical isolation of B.
pertussis
. First, the isolation rates of B.
pertussis
were compared between direct inoculation of nasopharyngeal specimens and inoculation after being stored in Amies transport medium for eight hours or less. The organism was detected in 43 specimens from 29 patients with at least one of the two inoculation methods. The comparative isolation rates were 81% (35 of 43) for both direct inoculation and transport medium. Second, nasopharyngeal specimens were incubated for five days at 35 degrees C on the three media; Bordet Gengou Medium (BG) with 5 micrograms of
CEX
per ml, Cyclodextrin Solid Medium (CSM) with 5 micrograms of
CEX
per ml, and Charcoal Agar with 40 micrograms of
CEX
per ml. The organism was detected from 44 nasopharyngeal specimens of 20 patients on at least one of the three tested media. The comparative isolation rates were 91% (40 of 44) on BG with 5 micrograms of
CEX
per ml, 93% (41 of 44) on CSM with 5 micrograms of
CEX
per ml, and 91% (40 of 44) on CA with 40 micrograms of
CEX
per ml. Although no differences in the isolation rates were observed among the three media, the appearance of the colonies was earlier by one day on BG than the other two media. The detection of B.
pertussis
was occasionally easier on CA than on the other two because of its higher suppression of nasopharyngeal flora.(ABSTRACT TRUNCATED AT 250 WORDS)
...
PMID:Simple and efficient method for clinical isolation of Bordetella pertussis. 177 12
Commercially available Amies transport medium with charcoal and three isolation media were tested to assess their efficiency in the clinical isolation of B.
pertussis
. First, the isolation rates of B.
pertussis
were compared between direct inoculation of nasopharyngeal specimens and inoculation after stored in Amies transport medium for 8 hours or less. The comparative isolation rates were 81% both (35 of 43 specimens from 29 patients) for direct inoculation and transport medium. Second, nasopharyngeal specimens were incubated for 5 days at 35 degrees C on the three media; Bordet-Gengou Medium (BG) with 5 micrograms of
CEX
per ml, Cyclodextrin Solid Medium (CSM) with 5 micrograms of
CEX
per ml, and Charcoal Agar with 40 micrograms of
CEX
per ml. The organism was detected from 44 nasopharyngeal specimens from 20 patients on at least one of the three tested media. The comparative isolation rates were 91% (40 of 44) on BG with 5 micrograms of
CEX
per ml, 93% (41 of 44) on CSM with 5 micrograms of
CEX
per ml, and 91% (40 of 44) on CA with 40 micrograms of
CEX
per ml. Although no differences in the isolation rates were observed among the three media, the appearance of the colonies was earlier by one day on BG than the rest of the two media. The detection of B.
pertussis
was occasionally easier on CA than the rest of the two because of its higher suppression for nasopharyngeal flora. CSM has its advantage in that it does not need any blood and can be prepared at anytime. Also, the shelf life of these three media proved to be at least one month when stored at 10 degrees C. We conclude that the clinical isolation of B.
pertussis
was highly successful with the following simple procedure: nasopharyngeal specimens stored in Amies transport medium and inoculated on one of the three media, BG, CSM, or CA, and then incubated for 5 days at 35 degrees C.
...
PMID:[Simple and efficient method for clinical isolation of Bordetella pertussis]. 207 51
