Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: UMLS:C0043167 (pertussis)
 19,595 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The invasive behaviour of 8 lymphoma cell lines were tested by an in vitro monolayer invasion assay. The metastatic cell lines (TAM 4D1.2, DCH10Sp, TAM 4D6.2, E4 and BWLi) were more invasive than their non-metastatic counterparts (TAS 5C4, BWO and DCH 10). There was a positive correlation between their invasiveness and the PGE1- and forskolin stimulated cellular cAMP levels. Invasiveness and basal cAMP levels could not be correlated. Pretreatment with pertussis toxin (50 ng/ml) for 24 hours provoked did not significantly affect the basal and PGE1-stimulated cAMP levels in all cells. Yet, the toxin catalysed the ADP-ribosylation of 40 kDa components in all cells and provoked a significant increase in the invasiveness of non-metastatic cell lines and a decrease in the invasiveness of metastatic cell lines. These data suggest that the invasiveness of T-lymphoma cell lines might be controlled by a complex interplay between different signal transducing pathways in the membrane, rather than by the intracellular level of cAMP.
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PMID:Cyclic AMP content and invasive capacity of metastatic variants of the BW-5147 murine T-cell lymphoma. 215 61

Skin allografting was performed in rats treated with cyclosporine using strain combinations that differed across the RT1.A (class I) or RT1.B (class II) loci of the major histocompatibility complex (MHC) or across non-MHC loci. Injection of cyclosporine (20 mg/kg) for 14 consecutive days was followed by prolonged acceptance (MST = 67 days) of the skin allografts. Bordetella pertussis vaccine potentiated the effect of cyclosporine, and the synergistic effect of the vaccine occurred only when it was given before grafting. The cyclosporine given for 14 days with or without B pertussis failed to prolong second-set skin grafts. Production of hemagglutinating antibodies in cyclosporine-treated animals was completely suppressed when the skin grafts were in place, and it occurred to a much lesser extent following rejection, as compared with untreated animals. The cyclosporine did not, however, influence the antibody response following second set skin graft rejection. A system in which rats were treated for only 6 days with cyclosporine was developed in order to test the effects of disparities at different histocompatibility loci on the response to cyclosporine, because this regimen gave a marginal, but significant, prolongation of skin grafts across a full RT1 (MHC) difference. There was a differential effect of cyclosporine treatment depending upon the genetic differences involved: a skin graft across an RT1.A (class I) difference was indefinitely prolonged, one across an RT1.B (class II) or RT1.AB difference was slightly prolonged (9 to 12 days and 12.5 to 16.5 days, respectively) and a graft across non-MHC differences was not affected. Hence, the immunosuppressive effects of cyclosporine on skin graft rejection appear to depend upon the genetic disparities between donor and recipient. Exploitation of this finding may lead to the design of more effective, multidrug protocols for the treatment of rejection that would have fewer deleterious side-effects.
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PMID:Prolongation of skin graft survival across different genetic barriers in rats with cyclosporine--and its potentiation by Bordetella pertussis vaccine. 634 41